Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus

Tsutomu Sasaki, Kazuo Kitagawa, Yoshiki Yagita, Shiro Sugiura, Emi Omura-Matsuoka, Shigeru Tanaka, Kohji Matsushita, Hideyuki Okano, Yoshihide Tsujitnoto, Masatsugu Hori

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Neuronal progenitors in the adult hippocampus continually proliferate and differentiate to the neuronal lineage, and ischemic insult promotes hippocampal neurogenesis. However, newborn neurons show a progressive reduction in numbers during the initial few weeks, therefore, enhanced survival of newborn neurons seems to be essential for therapeutic strategy. Bcl-2 is a crucial regulator of programmed cell death in CNS development and in apoptotic and necrotic cell death. Therefore, we tested whether Bcl-2 overexpression enhances survival of newborn neurons in the adult mouse hippocampus under normal and ischemic conditions. Many newborn neurons in the hippocampal dentate gyrus undergo apoptosis. Human Bcl-2 expression in NSE-bcl-2 transgenic mice began at the immature neuronal stage and remained constant in surviving mature neurons. Bcl-2 significantly increased survival of newborn neurons under both conditions, but particularly after ischemia, with decreased cell death of newborn neurons in NSE-bcl-2 transgenic mice. We also clarified the effect by Bcl-2 overexpression of enhanced survival of newborn neurons in primary hippocampal cultures with BrdU labeling. These findings suggest that Bcl-2 plays a crucial role in adult hippocampal neurogenesis under normal and ischemic conditions.

Original languageEnglish
Pages (from-to)1187-1196
Number of pages10
JournalJournal of Neuroscience Research
Volume84
Issue number6
DOIs
Publication statusPublished - 2006 Nov 1

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Hippocampus
Neurons
Cell Death
Neurogenesis
Transgenic Mice
Parahippocampal Gyrus
Dentate Gyrus
Bromodeoxyuridine
Ischemia
Apoptosis

Keywords

  • Bcl-2
  • Hippocampus
  • Ischemia
  • Neurogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sasaki, T., Kitagawa, K., Yagita, Y., Sugiura, S., Omura-Matsuoka, E., Tanaka, S., ... Hori, M. (2006). Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus. Journal of Neuroscience Research, 84(6), 1187-1196. https://doi.org/10.1002/jnr.21036

Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus. / Sasaki, Tsutomu; Kitagawa, Kazuo; Yagita, Yoshiki; Sugiura, Shiro; Omura-Matsuoka, Emi; Tanaka, Shigeru; Matsushita, Kohji; Okano, Hideyuki; Tsujitnoto, Yoshihide; Hori, Masatsugu.

In: Journal of Neuroscience Research, Vol. 84, No. 6, 01.11.2006, p. 1187-1196.

Research output: Contribution to journalArticle

Sasaki, T, Kitagawa, K, Yagita, Y, Sugiura, S, Omura-Matsuoka, E, Tanaka, S, Matsushita, K, Okano, H, Tsujitnoto, Y & Hori, M 2006, 'Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus', Journal of Neuroscience Research, vol. 84, no. 6, pp. 1187-1196. https://doi.org/10.1002/jnr.21036
Sasaki T, Kitagawa K, Yagita Y, Sugiura S, Omura-Matsuoka E, Tanaka S et al. Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus. Journal of Neuroscience Research. 2006 Nov 1;84(6):1187-1196. https://doi.org/10.1002/jnr.21036
Sasaki, Tsutomu ; Kitagawa, Kazuo ; Yagita, Yoshiki ; Sugiura, Shiro ; Omura-Matsuoka, Emi ; Tanaka, Shigeru ; Matsushita, Kohji ; Okano, Hideyuki ; Tsujitnoto, Yoshihide ; Hori, Masatsugu. / Bcl2 enhances survival of newborn neurons in the normal and ischemic hippocampus. In: Journal of Neuroscience Research. 2006 ; Vol. 84, No. 6. pp. 1187-1196.
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