Bead-based rapid construction of heterogeneous 3D tissue architecture

Yukiko Tsuda, Hiroaki Onoe, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This paper describes a method to construct macroscopic heterogeneous 3D tissue architecture by molding bead based micro-tissue units, so called "cell beads". We prepared mass-producible monodisperse collagen micro-gel beads coated with cells that we could stack into a mold to produce large-scale tissues rapidly with uniform cell density (Fig. 1). By using different types of cell beads or assembling macroscopic tissues prepared from the different cell types of beads, heterogeneous 3D tissue architecture was easily prepared. This approach would enable the rapid and reproducible construction of large-scale 3D tissues with a complex microstructure.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages2014-2016
Number of pages3
Volume3
Publication statusPublished - 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Fingerprint

Tissue
Collagen
Molding
Gels
Microstructure

Keywords

  • 3D tissue
  • Co-culture system
  • Collagen
  • Micro-beads
  • Tissue engineering

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Tsuda, Y., Onoe, H., & Takeuchi, S. (2010). Bead-based rapid construction of heterogeneous 3D tissue architecture. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 3, pp. 2014-2016)

Bead-based rapid construction of heterogeneous 3D tissue architecture. / Tsuda, Yukiko; Onoe, Hiroaki; Takeuchi, Shoji.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. p. 2014-2016.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuda, Y, Onoe, H & Takeuchi, S 2010, Bead-based rapid construction of heterogeneous 3D tissue architecture. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 3, pp. 2014-2016, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10/10/3.
Tsuda Y, Onoe H, Takeuchi S. Bead-based rapid construction of heterogeneous 3D tissue architecture. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3. 2010. p. 2014-2016
Tsuda, Yukiko ; Onoe, Hiroaki ; Takeuchi, Shoji. / Bead-based rapid construction of heterogeneous 3D tissue architecture. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 3 2010. pp. 2014-2016
@inproceedings{788f6a0b3e3c4da48d6336ea2c867d11,
title = "Bead-based rapid construction of heterogeneous 3D tissue architecture",
abstract = "This paper describes a method to construct macroscopic heterogeneous 3D tissue architecture by molding bead based micro-tissue units, so called {"}cell beads{"}. We prepared mass-producible monodisperse collagen micro-gel beads coated with cells that we could stack into a mold to produce large-scale tissues rapidly with uniform cell density (Fig. 1). By using different types of cell beads or assembling macroscopic tissues prepared from the different cell types of beads, heterogeneous 3D tissue architecture was easily prepared. This approach would enable the rapid and reproducible construction of large-scale 3D tissues with a complex microstructure.",
keywords = "3D tissue, Co-culture system, Collagen, Micro-beads, Tissue engineering",
author = "Yukiko Tsuda and Hiroaki Onoe and Shoji Takeuchi",
year = "2010",
language = "English",
isbn = "9781618390622",
volume = "3",
pages = "2014--2016",
booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

}

TY - GEN

T1 - Bead-based rapid construction of heterogeneous 3D tissue architecture

AU - Tsuda, Yukiko

AU - Onoe, Hiroaki

AU - Takeuchi, Shoji

PY - 2010

Y1 - 2010

N2 - This paper describes a method to construct macroscopic heterogeneous 3D tissue architecture by molding bead based micro-tissue units, so called "cell beads". We prepared mass-producible monodisperse collagen micro-gel beads coated with cells that we could stack into a mold to produce large-scale tissues rapidly with uniform cell density (Fig. 1). By using different types of cell beads or assembling macroscopic tissues prepared from the different cell types of beads, heterogeneous 3D tissue architecture was easily prepared. This approach would enable the rapid and reproducible construction of large-scale 3D tissues with a complex microstructure.

AB - This paper describes a method to construct macroscopic heterogeneous 3D tissue architecture by molding bead based micro-tissue units, so called "cell beads". We prepared mass-producible monodisperse collagen micro-gel beads coated with cells that we could stack into a mold to produce large-scale tissues rapidly with uniform cell density (Fig. 1). By using different types of cell beads or assembling macroscopic tissues prepared from the different cell types of beads, heterogeneous 3D tissue architecture was easily prepared. This approach would enable the rapid and reproducible construction of large-scale 3D tissues with a complex microstructure.

KW - 3D tissue

KW - Co-culture system

KW - Collagen

KW - Micro-beads

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=84884337456&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884337456&partnerID=8YFLogxK

M3 - Conference contribution

SN - 9781618390622

VL - 3

SP - 2014

EP - 2016

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

ER -