TY - JOUR
T1 - Beating activity of heterokaryons between myocardial and non-myocardial cells in culture
AU - Goshima, Kiyota
AU - Kaneko, Hiroyuki
AU - Wakabayashi, Shigeo
AU - Masuda, Akira
AU - Matsui, Yoichiro
N1 - Funding Information:
The authors wish to thank Dr G. Matsumoto, ElectrotechnicalL aboratory, Dr T. Obinata, Chiba University, and Dr K. Takahashi, University of Tokyo, for the critical discussion of this work. Thanks are also due to Dr Y. Okada, Osaka University, for kindly supplying HVJ, and to Dr K. Yamada, J. Toyama, Y. Kameyamaa nd K. Hashino, Nagoya University, for facilitatingt he supply of experimentala nimals. This work was supportedb y grants( to K. G.) from the Ministry of Education, Science and Culture of Japan, and the Ministry of Health and Welfare of Japan.
PY - 1984/3
Y1 - 1984/3
N2 - Cultured mouse myocardial cells grown as monolayers fused upon treatment with HVJ (Sendai virus). The myocardial cells also fused with quail myocardial cells, neuroblastoma cells and non-excitable cells, such as KB cells. The beating activity of these heterokaryons was studied in the present work. Heterokaryons composed of myocardial cells from different species maintained spontaneous beating activity for 2 days or more. Those of one myocardial and one neuroblastoma cell maintained the activity for 22-26 h, while those of one myocardial and one non-excitable cell, such as KB cell, lost the activity within 2-4 h after addition of HVJ. Heterokaryons that had stopped spontaneous beating did not contract on application of electrical-field stimulation. The ration of non-myocardial cells in the heterokaryons increased in inverse proportion to the decrease in beating activity of the heterokaryons. Study of the rapid disappearance of beating activity in heterokaryons composed of one myocardial and one KB cell showed that both excitability of the cell membrane and myofibril organization were rapidly lost.
AB - Cultured mouse myocardial cells grown as monolayers fused upon treatment with HVJ (Sendai virus). The myocardial cells also fused with quail myocardial cells, neuroblastoma cells and non-excitable cells, such as KB cells. The beating activity of these heterokaryons was studied in the present work. Heterokaryons composed of myocardial cells from different species maintained spontaneous beating activity for 2 days or more. Those of one myocardial and one neuroblastoma cell maintained the activity for 22-26 h, while those of one myocardial and one non-excitable cell, such as KB cell, lost the activity within 2-4 h after addition of HVJ. Heterokaryons that had stopped spontaneous beating did not contract on application of electrical-field stimulation. The ration of non-myocardial cells in the heterokaryons increased in inverse proportion to the decrease in beating activity of the heterokaryons. Study of the rapid disappearance of beating activity in heterokaryons composed of one myocardial and one KB cell showed that both excitability of the cell membrane and myofibril organization were rapidly lost.
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U2 - 10.1016/0014-4827(84)90364-1
DO - 10.1016/0014-4827(84)90364-1
M3 - Article
C2 - 6698116
AN - SCOPUS:0021337969
SN - 0014-4827
VL - 151
SP - 148
EP - 159
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -