BH3-only protein Bim is associated with the degree of Helicobacter pylori-induced gastritis and is localized to the mitochondria of inflammatory cells in the gastric mucosa

Yuko Akazawa, Katsuya Matsuda, Hajime Isomoto, Kayoko Matsushima, Yoko Kido, Shigetoshi Urabe, Naoyuki Yamaghchi, Ken Ohnita, Fuminao Takeshima, Hisayoshi Kondo, Hitoshi Tsugawa, Hidekazu Suzuki, Joel Moss, Kazuhiko Nakao, Masahiro Nakashima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

BH3-only protein, Bim, is a pro-apoptotic protein that mediates mitochondria-dependent cell death. However, the role of Bim in Helicobacter pylori-associated gastritis remains unclear. This study aimed to assess the cellular localization of Bim and its possible role in H. pylori-induced gastritis. The study was conducted on biopsy specimens obtained from 80 patients who underwent upper gastrointestinal endoscopy (H. pylori-negative: n = 30, positive: n = 50). Association between Bim mRNA expression and severity of gastritis was evaluated and the localization of Bim was examined by immunofluorescence. Bim mRNA expression was positively correlated with the degree of gastritis, as defined by the Sydney system. Immunohistochemical analysis confirmed increased Bim expression in H. pylori-infected gastric mucosa compared with uninfected mucosa in both humans and mice. Bim localized in myeloperoxidase- and CD138-positive cells of H. pylori-infected lamina propria and submucosa of the gastric tract, indicating that this protein is predominantly expressed in neutrophils and plasma cells. In contrast, Bim did not localize in CD20-, CD3-, or CD68-positive cells. Bim was expressed in the mitochondria, where it was partially co-localized with activated Bax and cleaved-PARP. In conclusion, Bim is expressed in neutrophils and plasma cells in H. pylori-associated gastritis, where it may participate in the termination of inflammatory response by causing mitochondria-mediated apoptosis in specific leucocytes.

Original languageEnglish
Pages (from-to)553-562
Number of pages10
JournalInternational Journal of Medical Microbiology
Volume305
Issue number6
DOIs
Publication statusPublished - 2015 Sep 1

Fingerprint

Gastritis
Gastric Mucosa
Helicobacter pylori
Mitochondria
Proteins
Plasma Cells
Mucous Membrane
Neutrophils
Messenger RNA
Apoptosis Regulatory Proteins
Gastrointestinal Endoscopy
Peroxidase
Fluorescent Antibody Technique
Stomach
Leukocytes
Cell Death
Apoptosis
Biopsy

Keywords

  • Apoptosis
  • Bax
  • Bcl-2
  • BH3-only protein
  • Bim
  • Gastritis
  • Helicobacter pylori
  • Mitochondria

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

BH3-only protein Bim is associated with the degree of Helicobacter pylori-induced gastritis and is localized to the mitochondria of inflammatory cells in the gastric mucosa. / Akazawa, Yuko; Matsuda, Katsuya; Isomoto, Hajime; Matsushima, Kayoko; Kido, Yoko; Urabe, Shigetoshi; Yamaghchi, Naoyuki; Ohnita, Ken; Takeshima, Fuminao; Kondo, Hisayoshi; Tsugawa, Hitoshi; Suzuki, Hidekazu; Moss, Joel; Nakao, Kazuhiko; Nakashima, Masahiro.

In: International Journal of Medical Microbiology, Vol. 305, No. 6, 01.09.2015, p. 553-562.

Research output: Contribution to journalArticle

Akazawa, Y, Matsuda, K, Isomoto, H, Matsushima, K, Kido, Y, Urabe, S, Yamaghchi, N, Ohnita, K, Takeshima, F, Kondo, H, Tsugawa, H, Suzuki, H, Moss, J, Nakao, K & Nakashima, M 2015, 'BH3-only protein Bim is associated with the degree of Helicobacter pylori-induced gastritis and is localized to the mitochondria of inflammatory cells in the gastric mucosa', International Journal of Medical Microbiology, vol. 305, no. 6, pp. 553-562. https://doi.org/10.1016/j.ijmm.2015.07.002
Akazawa, Yuko ; Matsuda, Katsuya ; Isomoto, Hajime ; Matsushima, Kayoko ; Kido, Yoko ; Urabe, Shigetoshi ; Yamaghchi, Naoyuki ; Ohnita, Ken ; Takeshima, Fuminao ; Kondo, Hisayoshi ; Tsugawa, Hitoshi ; Suzuki, Hidekazu ; Moss, Joel ; Nakao, Kazuhiko ; Nakashima, Masahiro. / BH3-only protein Bim is associated with the degree of Helicobacter pylori-induced gastritis and is localized to the mitochondria of inflammatory cells in the gastric mucosa. In: International Journal of Medical Microbiology. 2015 ; Vol. 305, No. 6. pp. 553-562.
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abstract = "BH3-only protein, Bim, is a pro-apoptotic protein that mediates mitochondria-dependent cell death. However, the role of Bim in Helicobacter pylori-associated gastritis remains unclear. This study aimed to assess the cellular localization of Bim and its possible role in H. pylori-induced gastritis. The study was conducted on biopsy specimens obtained from 80 patients who underwent upper gastrointestinal endoscopy (H. pylori-negative: n = 30, positive: n = 50). Association between Bim mRNA expression and severity of gastritis was evaluated and the localization of Bim was examined by immunofluorescence. Bim mRNA expression was positively correlated with the degree of gastritis, as defined by the Sydney system. Immunohistochemical analysis confirmed increased Bim expression in H. pylori-infected gastric mucosa compared with uninfected mucosa in both humans and mice. Bim localized in myeloperoxidase- and CD138-positive cells of H. pylori-infected lamina propria and submucosa of the gastric tract, indicating that this protein is predominantly expressed in neutrophils and plasma cells. In contrast, Bim did not localize in CD20-, CD3-, or CD68-positive cells. Bim was expressed in the mitochondria, where it was partially co-localized with activated Bax and cleaved-PARP. In conclusion, Bim is expressed in neutrophils and plasma cells in H. pylori-associated gastritis, where it may participate in the termination of inflammatory response by causing mitochondria-mediated apoptosis in specific leucocytes.",
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T1 - BH3-only protein Bim is associated with the degree of Helicobacter pylori-induced gastritis and is localized to the mitochondria of inflammatory cells in the gastric mucosa

AU - Akazawa, Yuko

AU - Matsuda, Katsuya

AU - Isomoto, Hajime

AU - Matsushima, Kayoko

AU - Kido, Yoko

AU - Urabe, Shigetoshi

AU - Yamaghchi, Naoyuki

AU - Ohnita, Ken

AU - Takeshima, Fuminao

AU - Kondo, Hisayoshi

AU - Tsugawa, Hitoshi

AU - Suzuki, Hidekazu

AU - Moss, Joel

AU - Nakao, Kazuhiko

AU - Nakashima, Masahiro

PY - 2015/9/1

Y1 - 2015/9/1

N2 - BH3-only protein, Bim, is a pro-apoptotic protein that mediates mitochondria-dependent cell death. However, the role of Bim in Helicobacter pylori-associated gastritis remains unclear. This study aimed to assess the cellular localization of Bim and its possible role in H. pylori-induced gastritis. The study was conducted on biopsy specimens obtained from 80 patients who underwent upper gastrointestinal endoscopy (H. pylori-negative: n = 30, positive: n = 50). Association between Bim mRNA expression and severity of gastritis was evaluated and the localization of Bim was examined by immunofluorescence. Bim mRNA expression was positively correlated with the degree of gastritis, as defined by the Sydney system. Immunohistochemical analysis confirmed increased Bim expression in H. pylori-infected gastric mucosa compared with uninfected mucosa in both humans and mice. Bim localized in myeloperoxidase- and CD138-positive cells of H. pylori-infected lamina propria and submucosa of the gastric tract, indicating that this protein is predominantly expressed in neutrophils and plasma cells. In contrast, Bim did not localize in CD20-, CD3-, or CD68-positive cells. Bim was expressed in the mitochondria, where it was partially co-localized with activated Bax and cleaved-PARP. In conclusion, Bim is expressed in neutrophils and plasma cells in H. pylori-associated gastritis, where it may participate in the termination of inflammatory response by causing mitochondria-mediated apoptosis in specific leucocytes.

AB - BH3-only protein, Bim, is a pro-apoptotic protein that mediates mitochondria-dependent cell death. However, the role of Bim in Helicobacter pylori-associated gastritis remains unclear. This study aimed to assess the cellular localization of Bim and its possible role in H. pylori-induced gastritis. The study was conducted on biopsy specimens obtained from 80 patients who underwent upper gastrointestinal endoscopy (H. pylori-negative: n = 30, positive: n = 50). Association between Bim mRNA expression and severity of gastritis was evaluated and the localization of Bim was examined by immunofluorescence. Bim mRNA expression was positively correlated with the degree of gastritis, as defined by the Sydney system. Immunohistochemical analysis confirmed increased Bim expression in H. pylori-infected gastric mucosa compared with uninfected mucosa in both humans and mice. Bim localized in myeloperoxidase- and CD138-positive cells of H. pylori-infected lamina propria and submucosa of the gastric tract, indicating that this protein is predominantly expressed in neutrophils and plasma cells. In contrast, Bim did not localize in CD20-, CD3-, or CD68-positive cells. Bim was expressed in the mitochondria, where it was partially co-localized with activated Bax and cleaved-PARP. In conclusion, Bim is expressed in neutrophils and plasma cells in H. pylori-associated gastritis, where it may participate in the termination of inflammatory response by causing mitochondria-mediated apoptosis in specific leucocytes.

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KW - Helicobacter pylori

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