Bidirectional regulation of dopamine D2 and neurotensin NTS1 receptors in dopamine neurons

C. Jomphe, P. L. Lemelin, Hideyuki Okano, K. Kobayashi, L. E. Trudeau

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Several lines of evidence suggest a close association between dopamine (DA) and neurotensin (NT) systems in the CNS. Indeed, in the rodent brain, abundant NT-containing fibres are found in DA-rich areas such as the ventral tegmental area and substantia nigra. Moreover, it has been shown in vivo that NT, acting through its high-affinity receptor (NTS1), reduces the physiological and behavioural effects of DA D2 receptor (D2R) activation, a critical autoreceptor feedback system regulating DA neurotransmission. However, the mechanism of this interaction is still elusive. The aim of our study was thus to reproduce in vitro the interaction between D2R and NTS1, and then to characterize the mechanisms implicated. We used a primary culture model of DA neurons prepared from transgenic mice expressing green fluorescent protein under the control of the tyrosine hydroxylase promoter. In these cultures, DA neurons endogenously express both D2R and NTS1. Using electrophysiological recordings, we show that activation of D2R directly inhibits the firing rate of DA neurons. In addition, we find that NT, acting through a NTS1-like receptor, is able to reduce D2R autoreceptor function independently of its ability to enhance DA neuron firing, and that this interaction occurs through a protein kinase C- and Ca 2+-dependent mechanism. Furthermore, prior activation of D2R reduces the ability of NTS1 to induce intracellular Ca2+ mobilization. Our findings provide evidence for bidirectional interaction between D2R and NTS1 in DA neurons, a regulatory mechanism that could play a key role in the control of the activity of these neurons.

Original languageEnglish
Pages (from-to)2789-2800
Number of pages12
JournalEuropean Journal of Neuroscience
Volume24
Issue number10
DOIs
Publication statusPublished - 2006 Dec

Fingerprint

Neurotensin Receptors
Dopaminergic Neurons
Neurotensin
Dopamine
Autoreceptors
Ventral Tegmental Area
Dopamine D2 Receptors
Tyrosine 3-Monooxygenase
Substantia Nigra
Green Fluorescent Proteins
Synaptic Transmission
Protein Kinase C
Transgenic Mice
Rodentia
Neurons
Brain

Keywords

  • Culture
  • GPCR
  • Mice
  • Patch-clamp
  • Receptor interaction

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bidirectional regulation of dopamine D2 and neurotensin NTS1 receptors in dopamine neurons. / Jomphe, C.; Lemelin, P. L.; Okano, Hideyuki; Kobayashi, K.; Trudeau, L. E.

In: European Journal of Neuroscience, Vol. 24, No. 10, 12.2006, p. 2789-2800.

Research output: Contribution to journalArticle

Jomphe, C. ; Lemelin, P. L. ; Okano, Hideyuki ; Kobayashi, K. ; Trudeau, L. E. / Bidirectional regulation of dopamine D2 and neurotensin NTS1 receptors in dopamine neurons. In: European Journal of Neuroscience. 2006 ; Vol. 24, No. 10. pp. 2789-2800.
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