Bile acid binding resin prevents fat accumulation through intestinal microbiota in high-fat diet-induced obesity in mice

Yukie Kusumoto, Junichiro Irie, Kaho Iwabu, Hirotsune Tagawa, Arata Itoh, Mari Kato, Nana Kobayashi, Kumiko Tanaka, Rieko Kikuchi, Masataka Fujita, Yuya Nakajima, Kohkichi Morimoto, Taichi Sugizaki, Satoru Yamada, Toshihide Kawai, Mitsuhiro Watanabe, Yuichi Oike, Hiroshi Itoh

Research output: Contribution to journalArticle

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Abstract

Background Bile acid binding resin (BAR) absorbs intestinal bile acids, and improves obesity and metabolic disorders, but the precise mechanism remains to be clarified. Recent findings reveal that obesity is associated with skewed intestinal microbiota. Thus, we investigated the effect of BAR on intestinal microbiota and the role of microbiota in the prevention of obesity in high-fat diet-induced obesity in mice. Procedures Male Balb/c mice were fed a low-fat diet (LFD), high-fat diet (HFD), or HFD with BAR (HFD + BAR), and then metabolic parameters, caecal microbiota, and metabolites were investigated. The same interventions were conducted in germ-free and antibiotic-treated mice. Main findings The frequency of Clostridium leptum subgroup was higher in both HFD-fed and HFD + BAR-fed mice than in LFD-fed mice. The frequency of Bacteroides-Prevotella group was lower in HFD-fed mice than in LFD-fed mice, but the frequency was higher in HFD + BAR-fed mice than in HFD-fed mice. Caecal propionate was lower in HFD-fed mice than in LFD-fed mice, and higher in HFD + BAR-fed mice than in HFD-fed mice. HFD + BAR-fed mice showed lower adiposity than HFD-fed mice, and the reduction was not observed in germ-free or antibiotic-treated mice. Colonized germ-free mice showed a reduction in adiposity by BAR administration. Energy expenditure was lower in HFD-fed mice and higher in HFD + BAR-fed mice, but the increments induced by administration of BAR were not observed in antibiotic-treated mice. Conclusions Modulation of intestinal microbiota by BAR could be a novel therapeutic approach for obesity.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalMetabolism: Clinical and Experimental
Volume71
DOIs
Publication statusPublished - 2017 Jun 1

Fingerprint

High Fat Diet
Bile Acids and Salts
Obesity
Fats
Fat-Restricted Diet
Gastrointestinal Microbiome
Microbiota
Adiposity
Anti-Bacterial Agents
Prevotella
Bacteroides
Clostridium
Propionates

Keywords

  • Bile acid
  • Intestinal microbiota
  • Obesity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Bile acid binding resin prevents fat accumulation through intestinal microbiota in high-fat diet-induced obesity in mice. / Kusumoto, Yukie; Irie, Junichiro; Iwabu, Kaho; Tagawa, Hirotsune; Itoh, Arata; Kato, Mari; Kobayashi, Nana; Tanaka, Kumiko; Kikuchi, Rieko; Fujita, Masataka; Nakajima, Yuya; Morimoto, Kohkichi; Sugizaki, Taichi; Yamada, Satoru; Kawai, Toshihide; Watanabe, Mitsuhiro; Oike, Yuichi; Itoh, Hiroshi.

In: Metabolism: Clinical and Experimental, Vol. 71, 01.06.2017, p. 1-6.

Research output: Contribution to journalArticle

Kusumoto, Y, Irie, J, Iwabu, K, Tagawa, H, Itoh, A, Kato, M, Kobayashi, N, Tanaka, K, Kikuchi, R, Fujita, M, Nakajima, Y, Morimoto, K, Sugizaki, T, Yamada, S, Kawai, T, Watanabe, M, Oike, Y & Itoh, H 2017, 'Bile acid binding resin prevents fat accumulation through intestinal microbiota in high-fat diet-induced obesity in mice', Metabolism: Clinical and Experimental, vol. 71, pp. 1-6. https://doi.org/10.1016/j.metabol.2017.02.011
Kusumoto, Yukie ; Irie, Junichiro ; Iwabu, Kaho ; Tagawa, Hirotsune ; Itoh, Arata ; Kato, Mari ; Kobayashi, Nana ; Tanaka, Kumiko ; Kikuchi, Rieko ; Fujita, Masataka ; Nakajima, Yuya ; Morimoto, Kohkichi ; Sugizaki, Taichi ; Yamada, Satoru ; Kawai, Toshihide ; Watanabe, Mitsuhiro ; Oike, Yuichi ; Itoh, Hiroshi. / Bile acid binding resin prevents fat accumulation through intestinal microbiota in high-fat diet-induced obesity in mice. In: Metabolism: Clinical and Experimental. 2017 ; Vol. 71. pp. 1-6.
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abstract = "Background Bile acid binding resin (BAR) absorbs intestinal bile acids, and improves obesity and metabolic disorders, but the precise mechanism remains to be clarified. Recent findings reveal that obesity is associated with skewed intestinal microbiota. Thus, we investigated the effect of BAR on intestinal microbiota and the role of microbiota in the prevention of obesity in high-fat diet-induced obesity in mice. Procedures Male Balb/c mice were fed a low-fat diet (LFD), high-fat diet (HFD), or HFD with BAR (HFD + BAR), and then metabolic parameters, caecal microbiota, and metabolites were investigated. The same interventions were conducted in germ-free and antibiotic-treated mice. Main findings The frequency of Clostridium leptum subgroup was higher in both HFD-fed and HFD + BAR-fed mice than in LFD-fed mice. The frequency of Bacteroides-Prevotella group was lower in HFD-fed mice than in LFD-fed mice, but the frequency was higher in HFD + BAR-fed mice than in HFD-fed mice. Caecal propionate was lower in HFD-fed mice than in LFD-fed mice, and higher in HFD + BAR-fed mice than in HFD-fed mice. HFD + BAR-fed mice showed lower adiposity than HFD-fed mice, and the reduction was not observed in germ-free or antibiotic-treated mice. Colonized germ-free mice showed a reduction in adiposity by BAR administration. Energy expenditure was lower in HFD-fed mice and higher in HFD + BAR-fed mice, but the increments induced by administration of BAR were not observed in antibiotic-treated mice. Conclusions Modulation of intestinal microbiota by BAR could be a novel therapeutic approach for obesity.",
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T1 - Bile acid binding resin prevents fat accumulation through intestinal microbiota in high-fat diet-induced obesity in mice

AU - Kusumoto, Yukie

AU - Irie, Junichiro

AU - Iwabu, Kaho

AU - Tagawa, Hirotsune

AU - Itoh, Arata

AU - Kato, Mari

AU - Kobayashi, Nana

AU - Tanaka, Kumiko

AU - Kikuchi, Rieko

AU - Fujita, Masataka

AU - Nakajima, Yuya

AU - Morimoto, Kohkichi

AU - Sugizaki, Taichi

AU - Yamada, Satoru

AU - Kawai, Toshihide

AU - Watanabe, Mitsuhiro

AU - Oike, Yuichi

AU - Itoh, Hiroshi

PY - 2017/6/1

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N2 - Background Bile acid binding resin (BAR) absorbs intestinal bile acids, and improves obesity and metabolic disorders, but the precise mechanism remains to be clarified. Recent findings reveal that obesity is associated with skewed intestinal microbiota. Thus, we investigated the effect of BAR on intestinal microbiota and the role of microbiota in the prevention of obesity in high-fat diet-induced obesity in mice. Procedures Male Balb/c mice were fed a low-fat diet (LFD), high-fat diet (HFD), or HFD with BAR (HFD + BAR), and then metabolic parameters, caecal microbiota, and metabolites were investigated. The same interventions were conducted in germ-free and antibiotic-treated mice. Main findings The frequency of Clostridium leptum subgroup was higher in both HFD-fed and HFD + BAR-fed mice than in LFD-fed mice. The frequency of Bacteroides-Prevotella group was lower in HFD-fed mice than in LFD-fed mice, but the frequency was higher in HFD + BAR-fed mice than in HFD-fed mice. Caecal propionate was lower in HFD-fed mice than in LFD-fed mice, and higher in HFD + BAR-fed mice than in HFD-fed mice. HFD + BAR-fed mice showed lower adiposity than HFD-fed mice, and the reduction was not observed in germ-free or antibiotic-treated mice. Colonized germ-free mice showed a reduction in adiposity by BAR administration. Energy expenditure was lower in HFD-fed mice and higher in HFD + BAR-fed mice, but the increments induced by administration of BAR were not observed in antibiotic-treated mice. Conclusions Modulation of intestinal microbiota by BAR could be a novel therapeutic approach for obesity.

AB - Background Bile acid binding resin (BAR) absorbs intestinal bile acids, and improves obesity and metabolic disorders, but the precise mechanism remains to be clarified. Recent findings reveal that obesity is associated with skewed intestinal microbiota. Thus, we investigated the effect of BAR on intestinal microbiota and the role of microbiota in the prevention of obesity in high-fat diet-induced obesity in mice. Procedures Male Balb/c mice were fed a low-fat diet (LFD), high-fat diet (HFD), or HFD with BAR (HFD + BAR), and then metabolic parameters, caecal microbiota, and metabolites were investigated. The same interventions were conducted in germ-free and antibiotic-treated mice. Main findings The frequency of Clostridium leptum subgroup was higher in both HFD-fed and HFD + BAR-fed mice than in LFD-fed mice. The frequency of Bacteroides-Prevotella group was lower in HFD-fed mice than in LFD-fed mice, but the frequency was higher in HFD + BAR-fed mice than in HFD-fed mice. Caecal propionate was lower in HFD-fed mice than in LFD-fed mice, and higher in HFD + BAR-fed mice than in HFD-fed mice. HFD + BAR-fed mice showed lower adiposity than HFD-fed mice, and the reduction was not observed in germ-free or antibiotic-treated mice. Colonized germ-free mice showed a reduction in adiposity by BAR administration. Energy expenditure was lower in HFD-fed mice and higher in HFD + BAR-fed mice, but the increments induced by administration of BAR were not observed in antibiotic-treated mice. Conclusions Modulation of intestinal microbiota by BAR could be a novel therapeutic approach for obesity.

KW - Bile acid

KW - Intestinal microbiota

KW - Obesity

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