Bile acids induce monocyte differentiation toward interleukin-12 hypo-producing dendritic cells via a TGR5-dependent pathway

Riko Ichikawa, Tetsuro Takayama, Kazuaki Yoneno, Nobuhiko Kamada, Mina T. Kitazume, Hajime Higuchi, Katsuyoshi Matsuoka, Mitsuhiro Watanabe, Hiroshi Itoh, Takanori Kanai, Tadakazu Hisamatsu, Toshifumi Hibi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Dendritic cells (DCs) are known as antigen-presenting cells and play a central role in both innate and acquired immunity. Peripheral blood monocytes give rise to resident and recruited DCs in lymph nodes and non-lymphoid tissues. The ligands of nuclear hormone receptors can modulate DC differentiation and so influence various biological functions of DCs. The role of bile acids (BAs) as signalling molecules has recently become apparent, but the functional role of BAs in DC differentiation has not yet been elucidated. We show that DCs derived from human peripheral blood monocytes cultured with a BA produce lower levels of interleukin-12 (IL-12) and tumour necrosis factor-α in response to stimulation with commensal bacterial antigens. Stimulation through the nuclear receptor farnesoid X (FXR) did not affect the differentiation of DCs. However, DCs differentiated with the specific agonist for TGR5, a transmembrane BA receptor, showed an IL-12 hypo-producing phenotype. Expression of TGR5 could only be identified in monocytes and was rapidly down-regulated during monocyte differentiation to DCs. Stimulation with 8-bromoadenosine-cyclic AMP (8-Br-cAMP), which acts downstream of TGR5 signalling, also promoted differentiation into IL-12 hypo-producing DCs. These results indicate that BAs induce the differentiation of IL-12 hypo-producing DCs from monocytes via the TGR5-cAMP pathway.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalImmunology
Volume136
Issue number2
DOIs
Publication statusPublished - 2012 Jun

Fingerprint

Interleukin-12
Bile Acids and Salts
Dendritic Cells
Monocytes
Cytoplasmic and Nuclear Receptors
Cell Differentiation
Interleukin-12 Receptors
Bacterial Antigens
Adaptive Immunity
Antigen-Presenting Cells
Innate Immunity
Cyclic AMP
Tumor Necrosis Factor-alpha
Lymph Nodes
Ligands
Phenotype

Keywords

  • Bile acid
  • CAMP
  • Dendritic cell differentiation
  • Interleukin-12
  • TGR5

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Ichikawa, R., Takayama, T., Yoneno, K., Kamada, N., Kitazume, M. T., Higuchi, H., ... Hibi, T. (2012). Bile acids induce monocyte differentiation toward interleukin-12 hypo-producing dendritic cells via a TGR5-dependent pathway. Immunology, 136(2), 153-162. https://doi.org/10.1111/j.1365-2567.2012.03554.x

Bile acids induce monocyte differentiation toward interleukin-12 hypo-producing dendritic cells via a TGR5-dependent pathway. / Ichikawa, Riko; Takayama, Tetsuro; Yoneno, Kazuaki; Kamada, Nobuhiko; Kitazume, Mina T.; Higuchi, Hajime; Matsuoka, Katsuyoshi; Watanabe, Mitsuhiro; Itoh, Hiroshi; Kanai, Takanori; Hisamatsu, Tadakazu; Hibi, Toshifumi.

In: Immunology, Vol. 136, No. 2, 06.2012, p. 153-162.

Research output: Contribution to journalArticle

Ichikawa, R, Takayama, T, Yoneno, K, Kamada, N, Kitazume, MT, Higuchi, H, Matsuoka, K, Watanabe, M, Itoh, H, Kanai, T, Hisamatsu, T & Hibi, T 2012, 'Bile acids induce monocyte differentiation toward interleukin-12 hypo-producing dendritic cells via a TGR5-dependent pathway', Immunology, vol. 136, no. 2, pp. 153-162. https://doi.org/10.1111/j.1365-2567.2012.03554.x
Ichikawa, Riko ; Takayama, Tetsuro ; Yoneno, Kazuaki ; Kamada, Nobuhiko ; Kitazume, Mina T. ; Higuchi, Hajime ; Matsuoka, Katsuyoshi ; Watanabe, Mitsuhiro ; Itoh, Hiroshi ; Kanai, Takanori ; Hisamatsu, Tadakazu ; Hibi, Toshifumi. / Bile acids induce monocyte differentiation toward interleukin-12 hypo-producing dendritic cells via a TGR5-dependent pathway. In: Immunology. 2012 ; Vol. 136, No. 2. pp. 153-162.
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