Binding of ADAM28 to P-selectin glycoprotein ligand-1 enhances P-selectin-mediated leukocyte adhesion to endothelial cells

Masayuki Shimoda, Gakuji Hashimoto, Satsuki Mochizuki, Eiji Ikeda, Norihiro Nagai, Susumu Ishida, Yasunori Okada

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

ADAMs (a disintegrin and metalloproteinases) are a recently discovered gene family of multifunctional proteins with the disintegrin-like and metalloproteinase domains. To analyze the biological functions of ADAM28, we screened binding molecules to secreted-type ADAM28 (ADAM28s) by the yeast two-hybrid system and identified P-selectin glycoprotein ligand-1 (PSGL-1). Binding between the disintegrin-like domain of ADAM28s and the extracellular portion of PSGL-1 was determined by yeast two-hybrid assays, binding assays of the domain-specific recombinant ADAM28s species using PSGL-1 stable transfectants and leukocyte cell lines expressing native PSGL-1 (HL-60 cells and Jurkat cells), and co-immunolocalization and co-immunoprecipitation of the molecules in these cells. Incubation of HL-60 cells with recombinant ADAM28s enhanced the binding to P-selectin-coated wells and P-selectin-expressing endothelial cells. In addition, intravenous injection of ADAM28s-treated HL-60 cells increased their accumulation in the pulmonary microcirculation and alveolar spaces in a mouse model of endotoxin-induced inflammation. These data suggest a novel function that ADAM28s promotes PSGL-1/P-selectin-mediated leukocyte rolling adhesion to endothelial cells and subsequent infiltration into tissue spaces through interaction with PSGL-1 on leukocytes under inflammatory conditions.

Original languageEnglish
Pages (from-to)25864-25874
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number35
DOIs
Publication statusPublished - 2007 Aug 31

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P-Selectin
Endothelial cells
Leukocytes
Adhesion
Endothelial Cells
Disintegrins
HL-60 Cells
Two-Hybrid System Techniques
Metalloproteases
Yeast
Assays
Leukocyte Rolling
Microcirculation
Molecules
Jurkat Cells
Hybrid systems
Immunoprecipitation
Infiltration
Endotoxins
Intravenous Injections

ASJC Scopus subject areas

  • Biochemistry

Cite this

Binding of ADAM28 to P-selectin glycoprotein ligand-1 enhances P-selectin-mediated leukocyte adhesion to endothelial cells. / Shimoda, Masayuki; Hashimoto, Gakuji; Mochizuki, Satsuki; Ikeda, Eiji; Nagai, Norihiro; Ishida, Susumu; Okada, Yasunori.

In: Journal of Biological Chemistry, Vol. 282, No. 35, 31.08.2007, p. 25864-25874.

Research output: Contribution to journalArticle

Shimoda, Masayuki ; Hashimoto, Gakuji ; Mochizuki, Satsuki ; Ikeda, Eiji ; Nagai, Norihiro ; Ishida, Susumu ; Okada, Yasunori. / Binding of ADAM28 to P-selectin glycoprotein ligand-1 enhances P-selectin-mediated leukocyte adhesion to endothelial cells. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 35. pp. 25864-25874.
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