Binding of calmodulin to Nuf1p is required for karyogamy in Saccharomyces cerevisiae

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Abstract

The role of calmodulin (CaM) during mating in Saccharomyces cerevisiae was examined by using a set of Phe-to-Ala substitutions. We identified ten CaM mutants that exhibited significantly reduced mating efficiencies when crossed to a strain of the opposite mating type harboring the same CaM mutation. Most of the mating-defective CaM mutants were bilateral, i.e., they also exhibited mating defects, albeit minor ones, when crossed to the wild type. When strains carrying different bilateral CaM mutations were mated, the mating efficiencies recovered dramatically. We termed this phenomenon "intragenic mating complementation", and classified the mating-defective CaM mutations into two intragenic mating complementation groups. Two mutant alleles belonging to different groups showed minor defects in cell adhesion and cell fusion, but exhibited severe defects in karyogamy. CaM is known to bind to the essential spindle pole body component Nuf1p. This binding appears to be important for karyogamy because the nuf1C911R mutation, which impairs CaM-Nuf1p binding, resulted in a severe defect in karyogamy. Indeed, the two mating-defective CaM mutations were found to compromise formation of the CaM/Nuf1p complex, and the mating defects of these two CaM mutants were suppressible by a dominant, CaM-independent, mutation in NUF1. Taken together, these results suggest that loss of CaM binding to Nuf1p causes a defect in karyogamy, thereby inhibiting productive mating.

Original languageEnglish
Pages (from-to)649-657
Number of pages9
JournalMolecular Genetics and Genomics
Volume269
Issue number5
DOIs
Publication statusPublished - 2003 Aug 1
Externally publishedYes

Fingerprint

Calmodulin
Saccharomyces cerevisiae
Mutation
Spindle Pole Bodies
Cell Fusion
Cell Adhesion
Alleles

Keywords

  • Calmodulin
  • Intragenic mating complementation
  • Mating
  • NUF1/SPC110
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Genetics

Cite this

Binding of calmodulin to Nuf1p is required for karyogamy in Saccharomyces cerevisiae. / Okano, Hideyuki; Ohya, Y.

In: Molecular Genetics and Genomics, Vol. 269, No. 5, 01.08.2003, p. 649-657.

Research output: Contribution to journalArticle

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