Binding of ferritin molecules to a charged polypeptide layer of poly-1-benzyl-L-histidine

T. Furuno, H. Sasabe, K. M. Ulmer

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The binding of ferritin molecules to a charged polypeptide layer of poly-1- benzyl-L-histidine spread at the air-water interface has been studied. The films were transferred onto an alkylated silicon wafer and were observed with a scanning electron microscope without staining or metal shadowing. The purpose of the present study was to explore the formation of densely and/or regularly packed two- dimensional arrays of protein molecules by means of the Langmuir-Blodgett method. Compression of the ferritin-peptide film at the air-water interface was found to be highly effective in producing closely packed arrays of ferritin molecules. Control experiments with an uncharged interfacial film of poly-γ-benzyl-L- glutamate gave no evidence of binding or array formation.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalThin Solid Films
Volume180
Issue number1-2
DOIs
Publication statusPublished - 1989 Nov 21

    Fingerprint

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this