Binding of ferritin molecules to a charged polypeptide layer of poly-1-benzyl-L-histidine

Taiji Furuno, H. Sasabe, K. M. Ulmer

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

The binding of ferritin molecules to a charged polypeptide layer of poly-1- benzyl-L-histidine spread at the air-water interface has been studied. The films were transferred onto an alkylated silicon wafer and were observed with a scanning electron microscope without staining or metal shadowing. The purpose of the present study was to explore the formation of densely and/or regularly packed two- dimensional arrays of protein molecules by means of the Langmuir-Blodgett method. Compression of the ferritin-peptide film at the air-water interface was found to be highly effective in producing closely packed arrays of ferritin molecules. Control experiments with an uncharged interfacial film of poly-γ-benzyl-L- glutamate gave no evidence of binding or array formation.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalThin Solid Films
Volume180
Issue number1-2
DOIs
Publication statusPublished - 1989 Nov 21
Externally publishedYes

Fingerprint

histidine
Polypeptides
polypeptides
Ferritins
Peptides
Molecules
molecules
glutamates
Water
air
staining
Air
Silicon wafers
water
peptides
Compaction
Electron microscopes
electron microscopes
Metals
wafers

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this

Binding of ferritin molecules to a charged polypeptide layer of poly-1-benzyl-L-histidine. / Furuno, Taiji; Sasabe, H.; Ulmer, K. M.

In: Thin Solid Films, Vol. 180, No. 1-2, 21.11.1989, p. 23-30.

Research output: Contribution to journalArticle

Furuno, Taiji ; Sasabe, H. ; Ulmer, K. M. / Binding of ferritin molecules to a charged polypeptide layer of poly-1-benzyl-L-histidine. In: Thin Solid Films. 1989 ; Vol. 180, No. 1-2. pp. 23-30.
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