Binding of hemagglutinin and influenza virus to a peptide-conjugated lipid membrane

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hemagglutinin (HA) plays an important role in the first step of influenza virus (IFV) infection because it initiates the binding of the virus to the sialylgalactose linkages of the receptors on the host cells. We herein demonstrate that a HA-binding peptide immobilized on a solid support is available to bind to HA and IFV. We previously obtained a HA-binding pentapeptide (Ala-Arg-Leu-Pro-Arg), which was identified by phage-display selection against HAs from random peptide libraries. This peptide binds to the receptor-binding site of HA by mimicking sialic acid. A peptide-conjugated lipid (pep-PE) was chemically synthesized from the peptide and a saturated phospholipid. A lipid bilayer composed of pep-PE and an unsaturated phospholipid (DOPC) was immobilized on a mica plate; and the interaction between HA and the pep-PE/DOPC membrane was investigated using atomic force microscopy. The binding of IFV to the pep-PE/DOPC membrane was detected by an enzyme-linked immunosorbent assay and real-time reverse transcription PCR. Our results indicate that peptide-conjugated lipids are a useful molecular device for the detection of HA and IFV.

Original languageEnglish
Article number468
JournalFrontiers in Microbiology
Volume7
Issue numberAPR
DOIs
Publication statusPublished - 2016 Apr 7

Fingerprint

Hemagglutinins
Membrane Lipids
Orthomyxoviridae
Peptides
Phospholipids
Lipids
Virus Attachment
Peptide Library
Membranes
Atomic Force Microscopy
Lipid Bilayers
N-Acetylneuraminic Acid
Virus Diseases
Bacteriophages
Reverse Transcription
Enzyme-Linked Immunosorbent Assay
Binding Sites
Equipment and Supplies
Polymerase Chain Reaction
1,2-oleoylphosphatidylcholine

Keywords

  • Atomic force microscopy
  • Hemagglutinin
  • Influenza
  • Lipid bilayer
  • Peptide-conjugated lipid
  • Sialic acid-mimic peptide

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Binding of hemagglutinin and influenza virus to a peptide-conjugated lipid membrane. / Matsubara, Teruhiko; Shibata, Rabi; Sato, Toshinori.

In: Frontiers in Microbiology, Vol. 7, No. APR, 468, 07.04.2016.

Research output: Contribution to journalArticle

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