Binding of influenza A virus to monosialoganglioside (GM3) reconstituted in glucosylceramide and sphingomyelin membranes

Toshinori Sato, Takeshi Serizawa, Yoshio Okahata

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The binding of influenza A virus to GM3-containing monolayers at an air/water interface was quantitatively investigated by use of a quartz crystal microbalance (QCM). A QCM was horizontally attached to the monolayer from the air phase and the binding behavior of influenza virus was followed by the frequency changes of the QCM. GM3 was reconstituted in the monolayer of sphingomyelin (SM) or glucosylceramide (GlcCer). When the mole fraction of GM3 was below 30 mol%, the binding rate of the influenza A virus to the GM3/GlcCer membrane was significantly faster than that to GM3/SM membranes. When the mole fraction of GM3 in SM was below 20 mol%, specific binding of influenza virus was not observed at all. Binding of the virus to the GM3/GlcCer mixed membrane was inhibited by the addition of sialyllactose (Neu5Acα2-3Galβ1-4Glc). The virus binding was also visually observed by scanning electron microscopy. Viruses selectively bound to GM3/GlcCer (20:80, by mol%) membrane, but not to GM3/SM (20:80, by mol%) membrane. Furthermore, it was suggested that specific binding of influenza virus to the GM3/GlcCer membrane induced the changes in morphology of virus. It was clearly demonstrated that binding of influenza virus to GM3 was influenced by matrix lipids surrounding GM3.

Original languageEnglish
Pages (from-to)14-20
Number of pages7
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1285
Issue number1
DOIs
Publication statusPublished - 1996 Nov 13
Externally publishedYes

Fingerprint

Glucosylceramides
Sphingomyelins
Influenza A virus
Viruses
Quartz Crystal Microbalance Techniques
Orthomyxoviridae
Membranes
Virus Attachment
Quartz crystal microbalances
Monolayers
Air
Electron Scanning Microscopy
sialogangliosides
Lipids
Water

Keywords

  • Air
  • Ganglioside
  • Glucosylceramide
  • Influenza A virus
  • Quartz crystal microbalance
  • Sphingomyelin
  • Water interface monolayer

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Binding of influenza A virus to monosialoganglioside (GM3) reconstituted in glucosylceramide and sphingomyelin membranes. / Sato, Toshinori; Serizawa, Takeshi; Okahata, Yoshio.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1285, No. 1, 13.11.1996, p. 14-20.

Research output: Contribution to journalArticle

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