Binding of tetrakis(pyrazoliumyl)porphyrin and its copper(II) and zinc(II) complexes to poly(dG-dC)2 and poly(dA-dT)2

Daryono H. Tjahjono, Rahmana E. Kartasasmita, As'ari Nawawi, Shunsuke Mima, Takehiro Akutsu, Naoki Yoshioka, Hidenari Inoue

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Interactions of cationic porphyrins bearing five-membered rings at the meso position, meso-tetrakis(1,2-dimethylpyrazolium-4-yl)porphyrin (MPzP; M is H2, CuII or ZnII), with synthetic polynucleotides poly(dG-dC)2 and poly(dA-dT)2 have been characterized by viscometric, visible absorption, circular dichroisim and magnetic circular dichroism spectroscopic and melting temperature measurements. Both H2PzP and CuPzP are intercalated into poly(dG-dC)2 and are outside-bound to the major groove of poly(dA-dT)2, while ZnPzP is outside-bound to the minor groove of poly(dA-dT)2 and surprisingly is intercalated into poly(dG-dC)2. The binding constants of the porphyrin and poly(dG-dC)2 and poly(dA-dT)2 are on the order of 106 M-1 and are comparable to those of other cationic porphyrins so far reported. The process of the binding of the porphyrin to poly(dG-dC)2 and poly(dA-dT)2 is exothermic and enthalpically driven for H2PzP, whereas it is endothermic and entropically driven for CuPzP and ZnPzP. These results have revealed that the kind of the central metal ion of metalloporphyrins influences the characteristics of the binding of the porphyrins to DNA.

Original languageEnglish
Pages (from-to)527-538
Number of pages12
JournalJournal of Biological Inorganic Chemistry
Volume11
Issue number4
DOIs
Publication statusPublished - 2006 Jun 1

Keywords

  • Binding mode
  • Cationic porphyrin
  • DNA binding
  • Thermodynamics

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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