Bioassay-guided isolation of compounds from Datura stramonium with TRAIL-resistance overcoming activity

Utpal K. Karmakar; Kazufumi Toume; Naoki Ishikawa; Midori A. Arai; Samir K. Sadhu; Firoj Ahmed; Masami Ishibashi

doi:10.1177/1934578x1601100213

Bioassay-guided isolation of compounds from Datura stramonium with TRAIL-resistance overcoming activity

Utpal K. Karmakar, Kazufumi Toume, Naoki Ishikawa, Midori A. Arai, Samir K. Sadhu, Firoj Ahmed, Masami Ishibashi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

TRAIL is a potent inducer of apoptosis in most cancer cells, but not in normal cells, and therefore has deserved intense interest as a promising agent for cancer therapy. In the search for bioactive natural products for overcoming TRAIL-resistance, we previously reported a number of active compounds. In our screening program on natural resources targeting overcoming TRAIL-resistance, activity-guided fractionation of the MeOH extract of Datura stramonium leaves led to the isolation of three alkaloids - scopolamine (1), trigonelline (2), and tyramine (3). Compounds 1, 2, and 3 exhibited TRAIL-resistance overcoming activity at 50, 150, and 100 μM, respectively in TRAIL-resistant AGS cells.

Original language	English
Pages (from-to)	185-187
Number of pages	3
Journal	Natural product communications
Volume	11
Issue number	2
DOIs	https://doi.org/10.1177/1934578x1601100213
Publication status	Published - 2016 Feb
Externally published	Yes

Keywords

Alkaloids
Datura stramonium
Solanaceae
TRAIL-resistance

ASJC Scopus subject areas

Pharmacology
Plant Science
Drug Discovery
Complementary and alternative medicine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1177/1934578x1601100213

Cite this

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title = "Bioassay-guided isolation of compounds from Datura stramonium with TRAIL-resistance overcoming activity",

abstract = "TRAIL is a potent inducer of apoptosis in most cancer cells, but not in normal cells, and therefore has deserved intense interest as a promising agent for cancer therapy. In the search for bioactive natural products for overcoming TRAIL-resistance, we previously reported a number of active compounds. In our screening program on natural resources targeting overcoming TRAIL-resistance, activity-guided fractionation of the MeOH extract of Datura stramonium leaves led to the isolation of three alkaloids - scopolamine (1), trigonelline (2), and tyramine (3). Compounds 1, 2, and 3 exhibited TRAIL-resistance overcoming activity at 50, 150, and 100 μM, respectively in TRAIL-resistant AGS cells.",

keywords = "Alkaloids, Datura stramonium, Solanaceae, TRAIL-resistance",

author = "Karmakar, {Utpal K.} and Kazufumi Toume and Naoki Ishikawa and Arai, {Midori A.} and Sadhu, {Samir K.} and Firoj Ahmed and Masami Ishibashi",

note = "Funding Information: This study was supported by KAKENHI Grants Number 23102008 from MEXT, 26305001, 26293022, and 25870128 from JSPS, and the Tokyo Biochemical Research Foundation (TBRF).",

year = "2016",

month = feb,

doi = "10.1177/1934578x1601100213",

language = "English",

volume = "11",

pages = "185--187",

journal = "Natural Product Communications",

issn = "1934-578X",

publisher = "Natural Product Communications",

number = "2",

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T1 - Bioassay-guided isolation of compounds from Datura stramonium with TRAIL-resistance overcoming activity

AU - Karmakar, Utpal K.

AU - Toume, Kazufumi

AU - Ishikawa, Naoki

AU - Arai, Midori A.

AU - Sadhu, Samir K.

AU - Ahmed, Firoj

AU - Ishibashi, Masami

N1 - Funding Information: This study was supported by KAKENHI Grants Number 23102008 from MEXT, 26305001, 26293022, and 25870128 from JSPS, and the Tokyo Biochemical Research Foundation (TBRF).

PY - 2016/2

Y1 - 2016/2

N2 - TRAIL is a potent inducer of apoptosis in most cancer cells, but not in normal cells, and therefore has deserved intense interest as a promising agent for cancer therapy. In the search for bioactive natural products for overcoming TRAIL-resistance, we previously reported a number of active compounds. In our screening program on natural resources targeting overcoming TRAIL-resistance, activity-guided fractionation of the MeOH extract of Datura stramonium leaves led to the isolation of three alkaloids - scopolamine (1), trigonelline (2), and tyramine (3). Compounds 1, 2, and 3 exhibited TRAIL-resistance overcoming activity at 50, 150, and 100 μM, respectively in TRAIL-resistant AGS cells.

AB - TRAIL is a potent inducer of apoptosis in most cancer cells, but not in normal cells, and therefore has deserved intense interest as a promising agent for cancer therapy. In the search for bioactive natural products for overcoming TRAIL-resistance, we previously reported a number of active compounds. In our screening program on natural resources targeting overcoming TRAIL-resistance, activity-guided fractionation of the MeOH extract of Datura stramonium leaves led to the isolation of three alkaloids - scopolamine (1), trigonelline (2), and tyramine (3). Compounds 1, 2, and 3 exhibited TRAIL-resistance overcoming activity at 50, 150, and 100 μM, respectively in TRAIL-resistant AGS cells.

KW - Alkaloids

KW - Datura stramonium

KW - Solanaceae

KW - TRAIL-resistance

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DO - 10.1177/1934578x1601100213

M3 - Article

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SN - 1934-578X

VL - 11

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JO - Natural Product Communications

JF - Natural Product Communications

IS - 2

ER -

Bioassay-guided isolation of compounds from Datura stramonium with TRAIL-resistance overcoming activity

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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