Biochemical characterization of the very long-chain fatty acid elongase ELOVL7

Tatsuro Naganuma, Yuichiro Sato, Takayuki Sassa, Yusuke Ohno, Akio Kihara

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Very long-chain fatty acids (VLCFAs) have a variety of physiological functions and are related to numerous disorders. The key step of VLCFA elongation is catalyzed by members of the elongase family, ELOVLs. Mammals have seven ELOVLs (ELOVL1-7), yet none of them has been purified and analyzed. In the presented study we purified ELOVL7 and measured its activity by reconstituting it into proteoliposomes. Purified ELOVL7 exhibited high activity toward acyl-CoAs with C18 carbon chain length. The calculated K m values toward C18:3(n-3)-CoA and malonyl-CoA were both in the μM range. We also found that progression of the VLCFA cycle enhances ELOVL7 activity.

Original languageEnglish
Pages (from-to)3337-3341
Number of pages5
JournalFEBS Letters
Volume585
Issue number20
DOIs
Publication statusPublished - 2011 Oct 20
Externally publishedYes

Keywords

  • ELOVL
  • Elongase
  • Lipid
  • Membrane
  • Very long-chain fatty acid

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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