Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment

J. Nozaki, M. Aizawa, H. Uchida, K. Itatani, H. Suemasu, A. Nozue, I. Okada, M. Matsumoto, H. Matsumoto, Y. Toyama

Research output: Contribution to journalConference articlepeer-review

3 Citations (Scopus)


HAp films with good adhesive property were formed by a simple surface treatment utilizing an enzyme reaction of urea with urease. The resulting coating layer consisted of carbonate-containing HAp with poor crystallinity. The in vitro evaluation using osteoblastic cells showed that HAp-coated Ti substrate had a good cellular response, such as cell proliferation and differentiation into osteoblast. From the results of in vivo evaluation using rabbit models, the HAp-coated Ti substrate directly bonded to bones to form large amount of new bone around implant. It has been found from both the in vitro and in vivo evaluations that the present HAp-coated Ti substrate has excellent biocompatibility.

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalKey Engineering Materials
Publication statusPublished - 2003
EventProceedings of the 15th International Symposium on Ceramics in Medicine; The Annual Meeting of the International Society for Ceramics in Medicine - Sydney, NSW, Australia
Duration: 2002 Dec 42002 Dec 8


  • Biocompatibility
  • Coating
  • Enzyme Reaction
  • Hydroxyapatite
  • In vitro Evaluation
  • In vivo Evaluation
  • Osteoblast
  • Surface Treatment
  • Tissue Reaction

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering


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