Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment

J. Nozaki, M. Aizawa, H. Uchida, K. Itatani, H. Suemasu, A. Nozue, I. Okada, Morio Matsumoto, Hideo Matsumoto, Y. Toyama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

HAp films with good adhesive property were formed by a simple surface treatment utilizing an enzyme reaction of urea with urease. The resulting coating layer consisted of carbonate-containing HAp with poor crystallinity. The in vitro evaluation using osteoblastic cells showed that HAp-coated Ti substrate had a good cellular response, such as cell proliferation and differentiation into osteoblast. From the results of in vivo evaluation using rabbit models, the HAp-coated Ti substrate directly bonded to bones to form large amount of new bone around implant. It has been found from both the in vitro and in vivo evaluations that the present HAp-coated Ti substrate has excellent biocompatibility.

Original languageEnglish
Pages (from-to)603-606
Number of pages4
JournalKey Engineering Materials
Volume240-242
Publication statusPublished - 2003

Fingerprint

Urease
Durapatite
Biocompatibility
Hydroxyapatite
Urea
Metals
Bone
Substrates
Carbonates
Osteoblasts
Cell proliferation
Surface treatment
Adhesives
Enzymes
Coatings

Keywords

  • Biocompatibility
  • Coating
  • Enzyme Reaction
  • Hydroxyapatite
  • In vitro Evaluation
  • In vivo Evaluation
  • Osteoblast
  • Surface Treatment
  • Tissue Reaction

ASJC Scopus subject areas

  • Ceramics and Composites
  • Chemical Engineering (miscellaneous)

Cite this

Nozaki, J., Aizawa, M., Uchida, H., Itatani, K., Suemasu, H., Nozue, A., ... Toyama, Y. (2003). Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment. Key Engineering Materials, 240-242, 603-606.

Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment. / Nozaki, J.; Aizawa, M.; Uchida, H.; Itatani, K.; Suemasu, H.; Nozue, A.; Okada, I.; Matsumoto, Morio; Matsumoto, Hideo; Toyama, Y.

In: Key Engineering Materials, Vol. 240-242, 2003, p. 603-606.

Research output: Contribution to journalArticle

Nozaki, J, Aizawa, M, Uchida, H, Itatani, K, Suemasu, H, Nozue, A, Okada, I, Matsumoto, M, Matsumoto, H & Toyama, Y 2003, 'Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment', Key Engineering Materials, vol. 240-242, pp. 603-606.
Nozaki J, Aizawa M, Uchida H, Itatani K, Suemasu H, Nozue A et al. Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment. Key Engineering Materials. 2003;240-242:603-606.
Nozaki, J. ; Aizawa, M. ; Uchida, H. ; Itatani, K. ; Suemasu, H. ; Nozue, A. ; Okada, I. ; Matsumoto, Morio ; Matsumoto, Hideo ; Toyama, Y. / Biocompatibility of hydroxyapatite films formed on Ti metal by a simple urea and urease treatment. In: Key Engineering Materials. 2003 ; Vol. 240-242. pp. 603-606.
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