Biodegradable microsphere-mediated cell perforation in microfluidic channel using femtosecond laser

Atsuhiro Ishii, Kazumasa Ariyasu, Tatsuki Mitsuhashi, Dag Heinemann, Alexander Heisterkamp, Mitsuhiro Terakawa

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The use of small particles has expanded the capability of ultrashort pulsed laser optoinjection technology toward simultaneous treatment of multiple cells. The microfluidic platform is one of the attractive systems that has obtained synergy with laser-based technology for cell manipulation, including optoinjection. We have demonstrated the delivery of molecules into suspended-flowing cells in a microfluidic channel by using biodegradable polymer microspheres and a near-infrared femtosecond laser pulse. The use of polylactic-co-glycolic acid microspheres realized not only a higher optoinjection ratio compared to that with polylactic acid microspheres but also avoids optical damage to the microfluidic chip, which is attributable to its higher optical intensity enhancement at the localized spot under a microsphere. Interestingly, optoinjection ratios to nucleus showed a difference for adhered cells and suspended cells. The use of biodegradable polymer microspheres provides high throughput optoinjection; i.e., multiple cells can be treated in a short time, which is promising for various applications in cell analysis, drug delivery, and ex vivo gene transfection to bone marrow cells and stem cells without concerns about residual microspheres.

Original languageEnglish
Article number055001
JournalJournal of Biomedical Optics
Issue number5
Publication statusPublished - 2016 May 1


  • Biodegradable polymer
  • Femtosecond laser
  • Laser cell perforation
  • Microfluidics
  • Optoinjection

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering


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