Biodegradable polymers in chondrogenesis of human articular chondrocytes

Nasreen Banu, Yasmin Banu, Masamune Sakai, Tadahiko Mashino, Toshie Tsuchiya

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of this study was to evaluate the potential role of polyglycolic acid (PGA), poly(glycolic acid-ε-caprolactone) (PGCL), poly(l-lactic acid-glycolic acid) (PLGA), poly(l-lactic acid-ε-caprolactone, 75:25 (w/w)) [P(LA-CL)25], poly-ε-caprolactone (tetrabutoxy titanium) [PCL(Ti)], and fullerene C-60 dimalonic acid (DMA) in cartilage transplants. After 4 weeks of culture of human articular cartilage, the levels of cell proliferation and differentiation and the expression of cartilage-specific matrix genes were estimated. The relationship between cell differentiation and gap junction protein connexin 43 (Cx43) was also evaluated. All materials except PCL(Ti) retained cell proliferation activities similar to the controls. Cell differentiation levels from the highest to the lowest were in the following order: PGA >> PLGA > PGCL > Control = DMSO > P(LA-CL)25 = PCL(Ti) >> fullerene C-60 DMA. Expression of the collagen type II gene was selectively upregulated for PGA, PGCL, and PLGA and slightly increased for P(LA-CL)25 polymers but was downregulated for fullerene C-60 DMA. Aggrecan gene expression was strongest with PGA and was consistently expressed with other matrices, especially with PGCL and PLGA. However, the expression patterns of the connexin 43 gene were different from the former two genes. Multiple regression analysis revealed a high correlation between cartilage proteoglycans production and expression levels of these three genes.

Original languageEnglish
Pages (from-to)184-191
Number of pages8
JournalJournal of Artificial Organs
Volume8
Issue number3
DOIs
Publication statusPublished - 2005 Sep 1

Keywords

  • Biodegradable polymers
  • Connexin 43
  • Human articular chondrocytes
  • Matrix gene

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering
  • Cardiology and Cardiovascular Medicine

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