Biodistribution of immunoliposome labeled with Tc-99m in tumor xenografted mice

Naoto Kitamura, Naoyuki Shigematsu, Tadaki Nakahara, Momoe Kanoh, Jun Hashimoto, Etsuo Kunieda, Atsushi Kubo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: Immunoliposome (PEG, GAH, liposome; PGL), consisting of F(ab)2 fragment of monoclonal antibody, GAH and polyethyleneglycol- coated (PEGylated) liposome was provided. Immunoliposome, PGL was labeled with technetium-99m (Tc-99m) by two methods: labeling F(ab)2 fragment with Tc-99m; Tc-99m-PGL, and entrapping Tc-99m into liposome; PGL[Tc-99m]. The objective of this study was to compare the biodistribution of Tc-99m-PGL and PGL[Tc-99m] in human gastric cancer xenografted mice. Methods: Tc-99m-PGL, PGL[Tc-99m], and Tc-99m-entrapped liposome; Lipo[Tc-99m] were prepared. They were injected into human gastric cancer, MKN45, xenografted mice via the tail vein, and their biodistribution was studied. Results: No marked accumulation of either PGL[Tc-99m] or Lipo[Tc-99m] was observed in the stomach. The uptake of Tc-99m-PGL by the liver, spleen, and lung was higher than that by the tumor. On the other hand, the uptake of PGL[Tc-99m] by the lung and spleen was markedly lower as compared with that of Tc-99m-PGL; the accumulation of PGL[Tc-99m] was lower in the lung and higher in the spleen as compared with that of the tumor. Although the liver uptake of PGL[Tc-99m] was markedly decreased as compared with that of Tc-99m-PGL, it was higher than the uptake of the tumor. The Tc-99m-PGL was strongly taken up by the tumor, with a high level of incorporation also seen in the stomach. These findings suggest the need for further study of the labeling stability. Conclusions: PGL[Tc-99m] appears to show promise for high tumor uptake and retention. This is an important implication for the potential application of immunoliposomes entrapped with Re-186, instead of Tc-99m, in internal radiotherapy.

Original languageEnglish
Pages (from-to)149-153
Number of pages5
JournalAnnals of Nuclear Medicine
Volume23
Issue number2
DOIs
Publication statusPublished - 2009 Feb 1

Keywords

  • Biodistribution
  • Immunoliposome
  • Tc-99m

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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