Biodosimetry of heavy ions by interphase chromosome painting

M. Durante, T. Kawata, T. Nakano, S. Yamada, H. Tsujii

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We report measurements of chromosomal aberrations in peripheral blood lymphocytes from cancer patients undergoing radiotherapy treatment. Patients with cervix or esophageal cancer were treated with 10 MV X-rays produced at a LINAC accelerator, or high-energy carbon ions produced at the HIMAC accelerator at the National Institute for Radiological Sciences (NIRS) in Chiba. Blood samples were obtained before, during, and after the radiation treatment. Chromosomes were prematurely condensed by incubation in calyculin A. Aberrations in chromosomes 2 and 4 were scored after fluorescence in situ hybridization with whole-chromosome probes. Pre-treatment samples were exposed in vitro to X-rays, individual dose-response curves for the induction of chromosomal aberrations were determined, and used as calibration curves to calculate the effective whole-body dose absorbed during the treatment. This calculated dose, based on the calibration curve relative to the induction of reciprocal exchanges, has a sharp increase after the first few fractions of the treatment, then saturates at high doses. Although carbon ions are 2-3 times more effective than X-rays in tumor sterilization, the effective dose was similar to that of X-ray treatment. However, the frequency of complex-type chromosomal exchangeswas much higher for patients treated with carbon ions than X-ray.

Original languageEnglish
Pages (from-to)1653-1662
Number of pages10
JournalAdvances in Space Research
Volume22
Issue number12
DOIs
Publication statusPublished - 1998 Jan 1

ASJC Scopus subject areas

  • Aerospace Engineering
  • Astronomy and Astrophysics
  • Geophysics
  • Atmospheric Science
  • Space and Planetary Science
  • Earth and Planetary Sciences(all)

Fingerprint Dive into the research topics of 'Biodosimetry of heavy ions by interphase chromosome painting'. Together they form a unique fingerprint.

  • Cite this

    Durante, M., Kawata, T., Nakano, T., Yamada, S., & Tsujii, H. (1998). Biodosimetry of heavy ions by interphase chromosome painting. Advances in Space Research, 22(12), 1653-1662. https://doi.org/10.1016/S0273-1177(99)00030-7