Biodosimetry of heavy ions by interphase chromosome painting

M. Durante, T. Kawata, T. Nakano, S. Yamada, H. Tsujii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report measurements of chromosomal aberrations in peripheral blood lymphocytes from cancer patients undergoing radiotherapy treatment. Patients with cervix or esophageal cancer were treated with 10 MV X-rays produced at a LINAC accelerator, or high-energy carbon ions produced at the HIMAC accelerator at the National Institute for Radiological Sciences (NIRS) in Chiba. Blood samples were obtained before, during, and after the radiation treatment. Chromosomes were prematurely condensed by incubation in calyculin A. Aberrations in chromosomes 2 and 4 were scored after fluorescence in situ hybridization with whole-chromosome probes. Pre-treatment samples were exposed in vitro to X-rays, individual dose-response curves for the induction of chromosomal aberrations were determined, and used as calibration curves to calculate the effective whole-body dose absorbed during the treatment. This calculated dose, based on the calibration curve relative to the induction of reciprocal exchanges, has a sharp increase after the first few fractions of the treatment, then saturates at high doses. Although carbon ions are 2-3 times more effective than X-rays in tumor sterilization, the effective dose was similar to that of X-ray treatment. However, the frequency of complex-type chromosomal exchangeswas much higher for patients treated with carbon ions than X-ray.

Original languageEnglish
Pages (from-to)1653-1662
Number of pages10
JournalAdvances in Space Research
Volume22
Issue number12
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

chromosomes
Painting
Chromosomes
Heavy ions
chromosome
heavy ions
X rays
dosage
Aberrations
ion
aberration
x rays
Dosimetry
Carbon
blood
Particle accelerators
carbon
cancer
induction
Ions

ASJC Scopus subject areas

  • Space and Planetary Science
  • Astronomy and Astrophysics

Cite this

Durante, M., Kawata, T., Nakano, T., Yamada, S., & Tsujii, H. (1998). Biodosimetry of heavy ions by interphase chromosome painting. Advances in Space Research, 22(12), 1653-1662.

Biodosimetry of heavy ions by interphase chromosome painting. / Durante, M.; Kawata, T.; Nakano, T.; Yamada, S.; Tsujii, H.

In: Advances in Space Research, Vol. 22, No. 12, 1998, p. 1653-1662.

Research output: Contribution to journalArticle

Durante, M, Kawata, T, Nakano, T, Yamada, S & Tsujii, H 1998, 'Biodosimetry of heavy ions by interphase chromosome painting', Advances in Space Research, vol. 22, no. 12, pp. 1653-1662.
Durante M, Kawata T, Nakano T, Yamada S, Tsujii H. Biodosimetry of heavy ions by interphase chromosome painting. Advances in Space Research. 1998;22(12):1653-1662.
Durante, M. ; Kawata, T. ; Nakano, T. ; Yamada, S. ; Tsujii, H. / Biodosimetry of heavy ions by interphase chromosome painting. In: Advances in Space Research. 1998 ; Vol. 22, No. 12. pp. 1653-1662.
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