Abstract
This paper describes a fabrication method and driving property of a biohybrid device with an antagonistic pair of skeletal muscle tissues and a flexible substrate. Since two skeletal muscle tissues are symmetrically arranged with the flexible substrate as the central axis, the flexible substrate deforms according to differences in their tension. In the formation of the antagonistic pair of skeletal muscle tissues, we assembled myoblast-laden hydrogel sheets and cultured them to construct a single skeletal muscle tissue on each side of the flexible substrate. We confirmed that the skeletal muscle tissue on the flexible substrate had the fundamental morphology and function of skeletal muscle. Furthermore, we made the biohybrid device actuate with deformation of the flexible substrate by selective contractions of the skeletal muscle tissues. From the deformation of the flexible substrate, we estimated the contractile force of each skeletal muscle tissue in the biohybrid device using finite element analysis. This biohybrid device, composed of an antagonistic pair of skeletal muscle tissues and a flexible substrate, can potentially be used in biological studies and pharmacokinetic assays involving the antagonistic pair of skeletal muscles.
| Original language | English |
|---|---|
| Journal | Advanced Robotics |
| DOIs | |
| Publication status | Accepted/In press - 2019 Jan 1 |
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Keywords
- biofabrication
- Biohybrid robot
- contractile force
- spontaneous shrinkage
- tissue engineering
ASJC Scopus subject areas
- Software
- Control and Systems Engineering
- Human-Computer Interaction
- Hardware and Architecture
- Computer Science Applications
Cite this
Biohybrid device with antagonistic skeletal muscle tissue for measurement of contractile force. / Morimoto, Yuya; Onoe, Hiroaki; Takeuchi, Shoji.
In: Advanced Robotics, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biohybrid device with antagonistic skeletal muscle tissue for measurement of contractile force
AU - Morimoto, Yuya
AU - Onoe, Hiroaki
AU - Takeuchi, Shoji
PY - 2019/1/1
Y1 - 2019/1/1
N2 - This paper describes a fabrication method and driving property of a biohybrid device with an antagonistic pair of skeletal muscle tissues and a flexible substrate. Since two skeletal muscle tissues are symmetrically arranged with the flexible substrate as the central axis, the flexible substrate deforms according to differences in their tension. In the formation of the antagonistic pair of skeletal muscle tissues, we assembled myoblast-laden hydrogel sheets and cultured them to construct a single skeletal muscle tissue on each side of the flexible substrate. We confirmed that the skeletal muscle tissue on the flexible substrate had the fundamental morphology and function of skeletal muscle. Furthermore, we made the biohybrid device actuate with deformation of the flexible substrate by selective contractions of the skeletal muscle tissues. From the deformation of the flexible substrate, we estimated the contractile force of each skeletal muscle tissue in the biohybrid device using finite element analysis. This biohybrid device, composed of an antagonistic pair of skeletal muscle tissues and a flexible substrate, can potentially be used in biological studies and pharmacokinetic assays involving the antagonistic pair of skeletal muscles.
AB - This paper describes a fabrication method and driving property of a biohybrid device with an antagonistic pair of skeletal muscle tissues and a flexible substrate. Since two skeletal muscle tissues are symmetrically arranged with the flexible substrate as the central axis, the flexible substrate deforms according to differences in their tension. In the formation of the antagonistic pair of skeletal muscle tissues, we assembled myoblast-laden hydrogel sheets and cultured them to construct a single skeletal muscle tissue on each side of the flexible substrate. We confirmed that the skeletal muscle tissue on the flexible substrate had the fundamental morphology and function of skeletal muscle. Furthermore, we made the biohybrid device actuate with deformation of the flexible substrate by selective contractions of the skeletal muscle tissues. From the deformation of the flexible substrate, we estimated the contractile force of each skeletal muscle tissue in the biohybrid device using finite element analysis. This biohybrid device, composed of an antagonistic pair of skeletal muscle tissues and a flexible substrate, can potentially be used in biological studies and pharmacokinetic assays involving the antagonistic pair of skeletal muscles.
KW - biofabrication
KW - Biohybrid robot
KW - contractile force
KW - spontaneous shrinkage
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85060465749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060465749&partnerID=8YFLogxK
U2 - 10.1080/01691864.2019.1567382
DO - 10.1080/01691864.2019.1567382
M3 - Article
AN - SCOPUS:85060465749
JO - Advanced Robotics
JF - Advanced Robotics
SN - 0169-1864
ER -