Bioinformatic analysis of post-transcriptional regulation by uORF in human and mouse

Motomu Matsui, Nozomu Yachie, Yuki Okada, Rintaro Saito, Masaru Tomita

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

RNA decay is thought to exert an important influence on gene expression by maintaining a steady-state level of transcripts and/or by eliminating aberrant transcripts. However, the sequence elements which control such processes have not been determined. Upstream open reading frames (uORFs) in the transcripts of several genes are reported to control translational initiation by stalling ribosomes and thereby promote RNA decay. We therefore performed bioinformatic analysis of the tissue-wide expression profiles and mRNA half-life of transcripts containing uORFs in humans and mice to assess the relationship between RNA decay and the presence of uORFs in transcripts. The expression levels of transcripts containing uORF were markedly lower than those not containing uORF. Moreover, the half-life of the uORF-containing transcripts was also shorter. These results suggest that uORFs are sequence elements that down-regulate RNA transcripts via RNA decay mechanisms.

Original languageEnglish
Pages (from-to)4184-4188
Number of pages5
JournalFEBS Letters
Volume581
Issue number22
DOIs
Publication statusPublished - 2007 Sep 4

Keywords

  • 5′ Untranslated region
  • Kozak consensus
  • Post-transcription control
  • RNA decay
  • Upstream open reading frames (uORF)

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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