Biological characteristics of human uterine endometrial cancer variant cells selected for blood group H type 1 antigen: Adhesion to vascular endothelial cells

Kaneyuki Kubushiro, Yuichi Sakuma, Hiroshi Yamashita, Takeshi Fukuchi, Kouji Banno, Katsumi Tsukazaki, Shiro Nozawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The expression level of Lewisb (Leb) antigen was previously shown to correlate with the five-year survival rate of human endometrial cancer patients. Variant cell lines with high (SNG-S) and low (SNG-W) levels of cell surface Leb antigen were isolated from the heterogeneous endometrial cancer cell line SNG-II cells to characterize the biological significance of Leb antigen on endometrial cancer cells. SNG-S cells mainly expressed Leb, whereas SNG-W cells mainly expressed H type 1 carbohydrate antigen alternatively. In an in vitro system, SNG-W cells showed a greater capacity to adhere to cytokine-activated human umbilical vein endothelial cells. Their adhesion to human umbilical vein endothelial cells was partially inhibited by pretreatment of antibody specific for H type 1 carbohydrate antigen. These results suggested that the H type 1 carbohydrate antigen expressed on endometrial cancer cells is involved in the adhesion to endothelial cells.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalActa Histochemica et Cytochemica
Volume33
Issue number3
DOIs
Publication statusPublished - 2000 Jan 1

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Keywords

  • Adhesion
  • Adhesion assay
  • Endometrial cancer
  • H type 1 antigen
  • Lewis antigen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biochemistry
  • Physiology
  • Histology
  • Cell Biology

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