Biological effects of blocking blue and other visible light on the mouse retina

Toshio Narimatsu, Yoko Ozawa, Seiji Miyake, Shunsuke Kubota, Kenya Yuki, Norihiro Nagai, Kazuo Tsubota

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: To elucidate the biological effects of blocking fluorescent light on the retina using specific blocking materials. Methods: Seven- to 8-week-old BALB/c mice were divided into three groups and placed in one of the three boxes: one blocked ultraviolet and violet wavelengths of light (violet blockade), one blocked ultraviolet, violet, blue and some other visible wavelengths (blue-plus blockade), and one allowed most visible light to pass through (control). They were then exposed to a white fluorescent lamp for 1h at 5.65E-05mW/cm2/s. After treatment, the electroretinogram, retinal outer nuclear layer thickness and retinal outer segment length were measured. In addition, retinal apoptotic cells were quantified by TdT-mediated dUTP nick-end labelling assay and c-Fos messenger RNA, and protein levels were measured by real-time reverse-transcription polymerase chain reaction and immunoblot analyses, respectively. Results: The blue-plus blockade group retained a significantly better electroretinogram response following light exposure than the control or violet blockade groups. The blue-plus blockade group also exhibited greater outer nuclear layer thickness and greater outer-segment length, and fewer apoptotic cells after light exposure than the other groups. The c-Fos messenger RNA and protein levels were substantially reduced in the blue-plus blockade group and reduced to a lesser extent in the violet blockade group. Conclusions: The blockade of blue plus additional visible wavelengths of light was most effective in protecting the retina from light-induced damage. The blockade of violet light alone was also effective in reducing intracellular molecular responses, but these effects were not sufficient for attenuating retinal degeneration.

Original languageEnglish
Pages (from-to)555-563
Number of pages9
JournalClinical and Experimental Ophthalmology
Volume42
Issue number6
DOIs
Publication statusPublished - 2014

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Retina
Light
Retinal Photoreceptor Cell Outer Segment
Retinal Degeneration
Messenger RNA
Reverse Transcription
Proteins
Polymerase Chain Reaction

Keywords

  • Apoptosis
  • c-Fos
  • Light
  • Photoreceptor
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Medicine(all)

Cite this

Biological effects of blocking blue and other visible light on the mouse retina. / Narimatsu, Toshio; Ozawa, Yoko; Miyake, Seiji; Kubota, Shunsuke; Yuki, Kenya; Nagai, Norihiro; Tsubota, Kazuo.

In: Clinical and Experimental Ophthalmology, Vol. 42, No. 6, 2014, p. 555-563.

Research output: Contribution to journalArticle

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AU - Nagai, Norihiro

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