Biological role of lutein in the light-induced retinal degeneration

Mariko Sasaki, Kenya Yuki, Toshihide Kurihara, Seiji Miyake, Kosuke Noda, Saori Kobayashi, Susumu Ishida, Kazuo Tsubota, Yoko Ozawa

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Lutein, a xanthophyll of a carotenoid, is anticipated as a therapeutic product to prevent human eye diseases. However, its biological mechanism is still unclear. Here, we show the molecular mechanism of lutein's effect to reduce photodamage of the retina. We analyzed the light-exposed retinas of Balb/c mice given lutein-supplemented or normal diet. Visual function was measured by electroretinogram, and histological changes were observed. Immunohistochemical and immunoblot analyses were performed to analyze molecular mechanism. The reactive oxygen species induced in the retina was evaluated by fluorescent probes. In the mice after light exposure, reduction of a-wave and b-wave amplitudes in electroretinogram, indicating visual impairment, and thinning of the photoreceptor cell layer owing to apoptosis were both attenuated by lutein diet. Interestingly, γ-H2AX, a marker for double-strand breaks (DSBs) in DNA, was up-regulated in the photoreceptor cells after light exposure, but this increase was attenuated by lutein diet, suggesting that DSBs caused by photodamage contributed to the photoreceptor cell death and that this change was suppressed by lutein. Moreover, the expression of eyes absent (EYA), which promotes DNA repair and cell survival, was significantly up-regulated with lutein diet in the light-exposed retina. Therefore, lutein induced EYA for DNA repair, which could suppress DNA damage and photoreceptor cell apoptosis. Lutein reduced light-induced oxidative stress in the retina, which might contribute to promote DNA repair. The lutein-supplemented diet attenuated light-induced visual impairment by protecting the photoreceptor cells' DNA.

Original languageEnglish
Pages (from-to)423-429
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume23
Issue number5
DOIs
Publication statusPublished - 2012 May

Fingerprint

Lutein
Retinal Degeneration
Light
Photoreceptor Cells
Nutrition
Retina
Diet
DNA
DNA Repair
Repair
Vision Disorders
Cells
Xanthophylls
Apoptosis
Oxidative stress
Double-Stranded DNA Breaks
Eye Diseases
Cell death
Carotenoids
Fluorescent Dyes

Keywords

  • DNA damage
  • Light exposure
  • Oxidative stress
  • Photoreceptor
  • Retina
  • Visual function

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Biological role of lutein in the light-induced retinal degeneration. / Sasaki, Mariko; Yuki, Kenya; Kurihara, Toshihide; Miyake, Seiji; Noda, Kosuke; Kobayashi, Saori; Ishida, Susumu; Tsubota, Kazuo; Ozawa, Yoko.

In: Journal of Nutritional Biochemistry, Vol. 23, No. 5, 05.2012, p. 423-429.

Research output: Contribution to journalArticle

Sasaki, Mariko ; Yuki, Kenya ; Kurihara, Toshihide ; Miyake, Seiji ; Noda, Kosuke ; Kobayashi, Saori ; Ishida, Susumu ; Tsubota, Kazuo ; Ozawa, Yoko. / Biological role of lutein in the light-induced retinal degeneration. In: Journal of Nutritional Biochemistry. 2012 ; Vol. 23, No. 5. pp. 423-429.
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