Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity

Hisae Aoshima, Shuichi Yamana, Shigeo Nakamura, Tadahiko Mashino

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We evaluated the safety of water-soluble polymer-enwrapped fullerenes (PVP/fullerenes) as antioxidants in cosmetic and pharmaceutical preparations by studying the genotoxicity, phototoxicity, and pro-oxidant effects of these fullerenes. These materials were not mutagenic to any of the tested bacterial strains and did not induce chromosomal aberrations in cultured mammalian cells. The PVP/fullerenes did not exhibit cytotoxicity under ultraviolet or sham irradiation in the alternative phototoxicity test. Moreover, they did not show any pro-oxidant effect in the presence of Fe<sup>2+</sup> or Cu<sup>2+</sup>. Thus, we concluded that PVP/fullerenes are safe for use in cosmetic and pharmaceutical applications. This is the first study in which toxicity tests were performed on PVP/fullerenes.

Original languageEnglish
Pages (from-to)401-409
Number of pages9
JournalJournal of Toxicological Sciences
Volume35
Issue number3
DOIs
Publication statusPublished - 2010

Fingerprint

Mutagenicity Tests
Phototoxic Dermatitis
Fullerenes
Reactive Oxygen Species
Safety
Water
Cosmetics
Toxicity Tests
Cytotoxicity
Aberrations
Chromosome Aberrations
Pharmaceutical Preparations
Toxicity
Cultured Cells
Polymers
Antioxidants
Cells
Irradiation

Keywords

  • Fullerene
  • Genotoxicity
  • Phototoxicity
  • Pro-oxidant
  • Safety evaluation

ASJC Scopus subject areas

  • Toxicology
  • Medicine(all)

Cite this

Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity. / Aoshima, Hisae; Yamana, Shuichi; Nakamura, Shigeo; Mashino, Tadahiko.

In: Journal of Toxicological Sciences, Vol. 35, No. 3, 2010, p. 401-409.

Research output: Contribution to journalArticle

@article{b5054a288780487a8d1271c4c4e75928,
title = "Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity",
abstract = "We evaluated the safety of water-soluble polymer-enwrapped fullerenes (PVP/fullerenes) as antioxidants in cosmetic and pharmaceutical preparations by studying the genotoxicity, phototoxicity, and pro-oxidant effects of these fullerenes. These materials were not mutagenic to any of the tested bacterial strains and did not induce chromosomal aberrations in cultured mammalian cells. The PVP/fullerenes did not exhibit cytotoxicity under ultraviolet or sham irradiation in the alternative phototoxicity test. Moreover, they did not show any pro-oxidant effect in the presence of Fe2+ or Cu2+. Thus, we concluded that PVP/fullerenes are safe for use in cosmetic and pharmaceutical applications. This is the first study in which toxicity tests were performed on PVP/fullerenes.",
keywords = "Fullerene, Genotoxicity, Phototoxicity, Pro-oxidant, Safety evaluation",
author = "Hisae Aoshima and Shuichi Yamana and Shigeo Nakamura and Tadahiko Mashino",
year = "2010",
doi = "10.2131/jts.35.401",
language = "English",
volume = "35",
pages = "401--409",
journal = "Journal of Toxicological Sciences",
issn = "0388-1350",
publisher = "Japanese Society of Toxicological Sciences",
number = "3",

}

TY - JOUR

T1 - Biological safety of water-soluble fullerenes evaluated using tests for genotoxicity, phototoxicity, and pro-oxidant activity

AU - Aoshima, Hisae

AU - Yamana, Shuichi

AU - Nakamura, Shigeo

AU - Mashino, Tadahiko

PY - 2010

Y1 - 2010

N2 - We evaluated the safety of water-soluble polymer-enwrapped fullerenes (PVP/fullerenes) as antioxidants in cosmetic and pharmaceutical preparations by studying the genotoxicity, phototoxicity, and pro-oxidant effects of these fullerenes. These materials were not mutagenic to any of the tested bacterial strains and did not induce chromosomal aberrations in cultured mammalian cells. The PVP/fullerenes did not exhibit cytotoxicity under ultraviolet or sham irradiation in the alternative phototoxicity test. Moreover, they did not show any pro-oxidant effect in the presence of Fe2+ or Cu2+. Thus, we concluded that PVP/fullerenes are safe for use in cosmetic and pharmaceutical applications. This is the first study in which toxicity tests were performed on PVP/fullerenes.

AB - We evaluated the safety of water-soluble polymer-enwrapped fullerenes (PVP/fullerenes) as antioxidants in cosmetic and pharmaceutical preparations by studying the genotoxicity, phototoxicity, and pro-oxidant effects of these fullerenes. These materials were not mutagenic to any of the tested bacterial strains and did not induce chromosomal aberrations in cultured mammalian cells. The PVP/fullerenes did not exhibit cytotoxicity under ultraviolet or sham irradiation in the alternative phototoxicity test. Moreover, they did not show any pro-oxidant effect in the presence of Fe2+ or Cu2+. Thus, we concluded that PVP/fullerenes are safe for use in cosmetic and pharmaceutical applications. This is the first study in which toxicity tests were performed on PVP/fullerenes.

KW - Fullerene

KW - Genotoxicity

KW - Phototoxicity

KW - Pro-oxidant

KW - Safety evaluation

UR - http://www.scopus.com/inward/record.url?scp=77953305211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77953305211&partnerID=8YFLogxK

U2 - 10.2131/jts.35.401

DO - 10.2131/jts.35.401

M3 - Article

C2 - 20519849

AN - SCOPUS:77953305211

VL - 35

SP - 401

EP - 409

JO - Journal of Toxicological Sciences

JF - Journal of Toxicological Sciences

SN - 0388-1350

IS - 3

ER -