Biological significance of gene amplification in carcinogenesis.

M. Terada, H. Sakamoto, Y. Ohmura, H. Tsuruta, N. Akiyama, H. Sasaki, M. Katoh, Yutaka Hattori, T. Yoshida

Research output: Contribution to journalArticle

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Abstract

K-SAM gene was originally isolated as an amplified gene in a stomach cancer cell line by in-gel DNA renaturation method. K-SAM encodes a membrane receptor with tyrosine kinase and is often amplified in poorly differentiated type of stomach cancer, while c-ERBB-2 is often amplified in well differentiated type of stomach cancer. There are several forms of K-SAM mRNAs which are generated by alternative splicing, and two types of K-SAM protein without transmembrane region. The ligand of K-SAM is considered to be growth factor(s) belonging to fibroblast growth factor (FGF) or heparin binding growth factor (HBFG) family. We have also frequently found amplification of HST-1 or HSTF1 gene in esophageal cancer. HST-1 gene, originally found as a transforming gene, is located on human chromosome 11q13, and it locates 35 kbp apart from its related gene, INT-2. Neither of the genes was expressed even in cancer cells with the co-amplification. By cosmid walking, we have identified at least two genes, designated tentatively as EXP1 and EXP2, on the same amplicon as HST-1 and INT-2, and the mRNAs for EXP1 and EXP2 genes were increased in amounts proportional to the degree of amplification.

Original languageEnglish
Pages (from-to)371-380
Number of pages10
JournalPrincess Takamatsu symposia
Volume22
Publication statusPublished - 1991
Externally publishedYes

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Gene Amplification
Carcinogenesis
Genes
Stomach Neoplasms
Intercellular Signaling Peptides and Proteins
Cosmids
Messenger RNA
Fibroblast Growth Factors
Alternative Splicing
Receptor Protein-Tyrosine Kinases
Human Chromosomes
Esophageal Neoplasms
Oncogenes
Walking
Heparin
Gels
Ligands
Cell Line
Membranes
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Terada, M., Sakamoto, H., Ohmura, Y., Tsuruta, H., Akiyama, N., Sasaki, H., ... Yoshida, T. (1991). Biological significance of gene amplification in carcinogenesis. Princess Takamatsu symposia, 22, 371-380.

Biological significance of gene amplification in carcinogenesis. / Terada, M.; Sakamoto, H.; Ohmura, Y.; Tsuruta, H.; Akiyama, N.; Sasaki, H.; Katoh, M.; Hattori, Yutaka; Yoshida, T.

In: Princess Takamatsu symposia, Vol. 22, 1991, p. 371-380.

Research output: Contribution to journalArticle

Terada, M, Sakamoto, H, Ohmura, Y, Tsuruta, H, Akiyama, N, Sasaki, H, Katoh, M, Hattori, Y & Yoshida, T 1991, 'Biological significance of gene amplification in carcinogenesis.', Princess Takamatsu symposia, vol. 22, pp. 371-380.
Terada M, Sakamoto H, Ohmura Y, Tsuruta H, Akiyama N, Sasaki H et al. Biological significance of gene amplification in carcinogenesis. Princess Takamatsu symposia. 1991;22:371-380.
Terada, M. ; Sakamoto, H. ; Ohmura, Y. ; Tsuruta, H. ; Akiyama, N. ; Sasaki, H. ; Katoh, M. ; Hattori, Yutaka ; Yoshida, T. / Biological significance of gene amplification in carcinogenesis. In: Princess Takamatsu symposia. 1991 ; Vol. 22. pp. 371-380.
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AU - Sasaki, H.

AU - Katoh, M.

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AU - Yoshida, T.

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