Biological significance of gene amplification in carcinogenesis.

M. Terada, H. Sakamoto, Y. Ohmura, H. Tsuruta, N. Akiyama, H. Sasaki, M. Katoh, Y. Hattori, T. Yoshida

Research output: Contribution to journalReview articlepeer-review

5 Citations (Scopus)


K-SAM gene was originally isolated as an amplified gene in a stomach cancer cell line by in-gel DNA renaturation method. K-SAM encodes a membrane receptor with tyrosine kinase and is often amplified in poorly differentiated type of stomach cancer, while c-ERBB-2 is often amplified in well differentiated type of stomach cancer. There are several forms of K-SAM mRNAs which are generated by alternative splicing, and two types of K-SAM protein without transmembrane region. The ligand of K-SAM is considered to be growth factor(s) belonging to fibroblast growth factor (FGF) or heparin binding growth factor (HBFG) family. We have also frequently found amplification of HST-1 or HSTF1 gene in esophageal cancer. HST-1 gene, originally found as a transforming gene, is located on human chromosome 11q13, and it locates 35 kbp apart from its related gene, INT-2. Neither of the genes was expressed even in cancer cells with the co-amplification. By cosmid walking, we have identified at least two genes, designated tentatively as EXP1 and EXP2, on the same amplicon as HST-1 and INT-2, and the mRNAs for EXP1 and EXP2 genes were increased in amounts proportional to the degree of amplification.

Original languageEnglish
Pages (from-to)371-380
Number of pages10
JournalPrincess Takamatsu symposia
Publication statusPublished - 1991
Externally publishedYes


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