Biologically active human and mouse nerve growth factors secreted by the yeast Saccharomyces cerevisiae

Masafumi Nishizawa, Fumiko Ozawa, Takako Higashizaki, Keiko Hirai, Fumio Hishinuma

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Nerve growth factor (NGF) is a trophic agent that is essential for the development and survival of sympathetic and sensory nerves. A chemically-synthesized DNA fragment encoding human NGF (hNGF) and a cDNA encoding mouse NGF (mNGF) were engineered for expression in the yeast, Saccharomyces cerevisiae. Expression and secretion of hNGF and mNGF was attempted under the direction of the yeast PGK promoter and with various leader sequences. Among the leader sequences tested, that of the yeast α-factor successfully directed secretion of both hNGF and mNGF that were correctly processed. The content of the recombinant NGF (reNGF) in the culture supernatant was estimated to be 1 μg/ml. The yeast-produced reNGF was able to bind to NGF receptors in rat pheochromocytoma (PC12) cells as efficiently as the standard mNGF, and partially purified reNGF could induce neurite outgrowth of PC12 cells. Thus, we have demonstrated that biologically active human and mouse reNGF can be produced in yeast cells.

Original languageEnglish
Pages (from-to)624-630
Number of pages7
JournalApplied Microbiology and Biotechnology
Volume38
Issue number5
DOIs
Publication statusPublished - 1993 Feb 1

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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