Biophysical signals underlying myogenic responses in rat interlobular artery

Tsuneo Takenaka, Hiromichi Suzuki, Hirokazu Okada, Koichi Hayashi, Yuri Ozawa, Takao Saruta

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

To assess cellular mechanisms mediating myogenic responses of interlobular artery (ILA), experiments were performed with the use of isolated perfused hydronephrotic kidneys. ILAs were divided into 3 groups according to their basal diameters: proximal (>60 μm), intermediate (40 to 60 μm), and distal (<40 μm) ILAs. Myogenic responses were obtained by stepwise increase in perfusion pressure. Greater myogenic responsiveness was observed in ILAs with smaller diameters. Diltiazem (10 μmol/L) inhibited myogenic responses of all segments of ILAs. Furthermore, gadolinium (10 μmol/L), a mechanosensitive cation channel blocker, abolished myogenic responses of distal but not proximal ILA. In contrast, 2-nitro-4- carboxphenyl-N, N-diphenyl-carbamate (200 μmol/L), an inhibitor of phospholipase C, prevented myogenic responses of proximal but not distal ILA. Finally, basal proximal ILA diameters were increased by treatment with 50 nmol/L of staurosporine (P<0.05), and subsequent addition of thapsigargin (1 μmol/L) blocked myogenic contraction of proximal ILAs. Myogenic responses of intermediate ILAs exhibited characteristics between those of distal and proximal ILAs. Our data indicate that underlying mechanisms for myogenic responses differ in distinct segments of ILAs. The present results suggest that mechanosensitive cation channels are involved in myogenic constriction of distal ILAs. Finally, our findings provide evidence that the stimulation of phospholipase C mediates myogenic contraction of proximal ILAs.

Original languageEnglish
Pages (from-to)1060-1065
Number of pages6
JournalHypertension
Volume32
Issue number6
Publication statusPublished - 1998 Dec

Fingerprint

Arteries
Type C Phospholipases
Cations
Thapsigargin
Staurosporine
Carbamates
Diltiazem
Gadolinium
Constriction
Perfusion
Kidney
Pressure

Keywords

  • Calcium
  • Calcium channels
  • Membrane potential
  • Phospholipases
  • Protein kinase C

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Takenaka, T., Suzuki, H., Okada, H., Hayashi, K., Ozawa, Y., & Saruta, T. (1998). Biophysical signals underlying myogenic responses in rat interlobular artery. Hypertension, 32(6), 1060-1065.

Biophysical signals underlying myogenic responses in rat interlobular artery. / Takenaka, Tsuneo; Suzuki, Hiromichi; Okada, Hirokazu; Hayashi, Koichi; Ozawa, Yuri; Saruta, Takao.

In: Hypertension, Vol. 32, No. 6, 12.1998, p. 1060-1065.

Research output: Contribution to journalArticle

Takenaka, T, Suzuki, H, Okada, H, Hayashi, K, Ozawa, Y & Saruta, T 1998, 'Biophysical signals underlying myogenic responses in rat interlobular artery', Hypertension, vol. 32, no. 6, pp. 1060-1065.
Takenaka T, Suzuki H, Okada H, Hayashi K, Ozawa Y, Saruta T. Biophysical signals underlying myogenic responses in rat interlobular artery. Hypertension. 1998 Dec;32(6):1060-1065.
Takenaka, Tsuneo ; Suzuki, Hiromichi ; Okada, Hirokazu ; Hayashi, Koichi ; Ozawa, Yuri ; Saruta, Takao. / Biophysical signals underlying myogenic responses in rat interlobular artery. In: Hypertension. 1998 ; Vol. 32, No. 6. pp. 1060-1065.
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