Biosynthesis of proteoglycans by lens epithelial cells of cataractous mouse (Nakano strain)

Kiyoshi Nakazawa, Makoto Takehana, Shuzo Iwata

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The capsules (with epithelial cells attached) of lenses from normal and cataractous mice (Nakano strain) were biosynthetically labeled in vitro with radioactive precursors. The labeled macro-molecules were chromatographed on a Sepharose CL-4B column and analyzed by specific enzyme digestion. The incorporation of [3H]-proline and [3H]-glucosamine into macromolecules was comparable in the cataract and normal capsules, while that of [35S]-sulfate was reduced by 60% in the cataract capsules, indicating that the proteoglycan synthesis was specifically decreased in the cataract lens. Glycosaminoglycan analyses showed an increased synthesis of hyaluronic acid and decreased synthesis of heparan sulfate in the cataract capsules. It is possible that the alterations in the synthetic level and glycosaminoglycan components of proteoglycan affect the permeabilities of macromolecules to lens capsule and lead to cataract in Nakano mouse lens.

Original languageEnglish
Pages (from-to)609-618
Number of pages10
JournalExperimental Eye Research
Volume40
Issue number4
DOIs
Publication statusPublished - 1985 Apr

Keywords

  • Nakano mouse
  • collagen
  • hyaluronic acid
  • lens capsule
  • lens epithelial cells
  • proteoglycan

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Biosynthesis of proteoglycans by lens epithelial cells of cataractous mouse (Nakano strain)'. Together they form a unique fingerprint.

  • Cite this