Biphasic activation of the JAK/STAT pathway by angiotensin II in rat cardiomyocytes

Hiroaki Kodama, Keiichi Fukuda, Jing Pan, Shinji Makino, Motoaki Sano, Toshiyuki Takahashi, Shingo Hori, Satoshi Ogawa

Research output: Contribution to journalArticle

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Abstract

This study was designed to demonstrate the characteristic pattern of angiotensin II-induced JAK/STAT (indicating just another kinase/signal transducer and activator of transcription) activation in cultured rat cardiomyocytes by comparing it with leukemia inhibitory factor (LIF)-induced activation. Angiotensin II (10-7 mol/L) induced rapid phosphorylation of JAK2 and Tyk2, but not JAK1, and phosphorylated STAT1 and STAT2, but not STAT3, in the early stage up to 30 minutes. The time course of JAK/STAT activation by angiotensin II was apparently slower than that by LIF. Interestingly, angiotensin II phosphorylated STAT3 and rephosphorylated STAT1 in the late stage at 120 minutes. We also found that angiotensin II induced the formation of interferon-stimulating gene factor (ISGF) complexes biphasically, in the early stage at 15 to 30 minutes and in the late stage at 120 minutes, and that angiotensin II induced delayed activation of the sis- inducing factor (SIF) complex at 120 minutes. Formation of ISGF and SIF complexes in response to angiotensin II paralleled the phosphorylation pattern of STAT1 and STAT3 and was quite different from those obtained in response to LIF. The phosphorylation of STAT1 was suppressed by pretreatment with the angiotensin II type-1 (AT1) receptor antagonist CV11974, but the delayed addition of CV11974 failed to suppress phosphorylation of STAT3 at 120 minutes. In conclusion, angiotensin II-induced JAK/STAT activation in rat cardiomyocytes is biphasic and entirely different from LIF-induced activation.

Original languageEnglish
Pages (from-to)244-250
Number of pages7
JournalCirculation Research
Volume82
Issue number2
Publication statusPublished - 1998 Feb 9

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Transducers
Cardiac Myocytes
Angiotensin II
Phosphotransferases
Leukemia Inhibitory Factor
Phosphorylation
Transcriptional Activation
Interferons
sis Genes
Angiotensin II Type 1 Receptor Blockers
Genes

Keywords

  • Angiotensin II
  • Cardiac hypertrophy
  • Cardiomyocyte
  • JAK/STAT pathway
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Biphasic activation of the JAK/STAT pathway by angiotensin II in rat cardiomyocytes. / Kodama, Hiroaki; Fukuda, Keiichi; Pan, Jing; Makino, Shinji; Sano, Motoaki; Takahashi, Toshiyuki; Hori, Shingo; Ogawa, Satoshi.

In: Circulation Research, Vol. 82, No. 2, 09.02.1998, p. 244-250.

Research output: Contribution to journalArticle

Kodama, H, Fukuda, K, Pan, J, Makino, S, Sano, M, Takahashi, T, Hori, S & Ogawa, S 1998, 'Biphasic activation of the JAK/STAT pathway by angiotensin II in rat cardiomyocytes', Circulation Research, vol. 82, no. 2, pp. 244-250.
Kodama, Hiroaki ; Fukuda, Keiichi ; Pan, Jing ; Makino, Shinji ; Sano, Motoaki ; Takahashi, Toshiyuki ; Hori, Shingo ; Ogawa, Satoshi. / Biphasic activation of the JAK/STAT pathway by angiotensin II in rat cardiomyocytes. In: Circulation Research. 1998 ; Vol. 82, No. 2. pp. 244-250.
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