Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs

Shun Ichi Miyazaki, Michisuke Yuzaki, Ken Nakada, Hideki Shirakawa, Setsuko Nakanishi, Shinji Nakade, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

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Abstract

The concentration of cytoplasmic free calcium (Ca2+) increases in various stimulated cells in a wave (Ca2+ wave) and in periodic transients (Ca2+ oscillations). These phenomena are explained by inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) and Ca2+-induced Ca2+ release (CICR) from separate intracellular stores, but decisive evidence is lacking. A monoclonal antibody to the IP3 receptor inhibited both IICR and CICR upon injection of IP3 and Ca2+ into hamster eggs, respectively. The antibody completely blocked sperm-induced Ca2+ waves and Ca2+ oscillations. The results indicate that Ca2 release in fertilized hamster eggs is mediated solely by the IP3 receptor, and Ca2+-sensitized IICR, but not CICR, generates Ca2+ waves and Ca2+ oscillations.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalScience
Volume257
Issue number5067
Publication statusPublished - 1992 Jul 10
Externally publishedYes

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Inositol 1,4,5-Trisphosphate Receptors
Zygote
Cricetinae
Inositol 1,4,5-Trisphosphate
Antibodies
Eggs
Spermatozoa
Monoclonal Antibodies
Calcium
Injections

ASJC Scopus subject areas

  • General

Cite this

Miyazaki, S. I., Yuzaki, M., Nakada, K., Shirakawa, H., Nakanishi, S., Nakade, S., & Mikoshiba, K. (1992). Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs. Science, 257(5067), 251-255.

Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs. / Miyazaki, Shun Ichi; Yuzaki, Michisuke; Nakada, Ken; Shirakawa, Hideki; Nakanishi, Setsuko; Nakade, Shinji; Mikoshiba, Katsuhiko.

In: Science, Vol. 257, No. 5067, 10.07.1992, p. 251-255.

Research output: Contribution to journalArticle

Miyazaki, SI, Yuzaki, M, Nakada, K, Shirakawa, H, Nakanishi, S, Nakade, S & Mikoshiba, K 1992, 'Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs', Science, vol. 257, no. 5067, pp. 251-255.
Miyazaki SI, Yuzaki M, Nakada K, Shirakawa H, Nakanishi S, Nakade S et al. Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs. Science. 1992 Jul 10;257(5067):251-255.
Miyazaki, Shun Ichi ; Yuzaki, Michisuke ; Nakada, Ken ; Shirakawa, Hideki ; Nakanishi, Setsuko ; Nakade, Shinji ; Mikoshiba, Katsuhiko. / Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs. In: Science. 1992 ; Vol. 257, No. 5067. pp. 251-255.
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AU - Nakada, Ken

AU - Shirakawa, Hideki

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AU - Mikoshiba, Katsuhiko

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