Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs

Shun Ichi Miyazaki, Michisuke Yuzaki, Ken Nakada, Hideki Shirakawa, Setsuko Nakanishi, Shinji Nakade, Katsuhiko Mikoshiba

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Abstract

The concentration of cytoplasmic free calcium (Ca2+) increases in various stimulated cells in a wave (Ca2+ wave) and in periodic transients (Ca2+ oscillations). These phenomena are explained by inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) and Ca2+-induced Ca2+ release (CICR) from separate intracellular stores, but decisive evidence is lacking. A monoclonal antibody to the IP3 receptor inhibited both IICR and CICR upon injection of IP3 and Ca2+ into hamster eggs, respectively. The antibody completely blocked sperm-induced Ca2+ waves and Ca2+ oscillations. The results indicate that Ca2 release in fertilized hamster eggs is mediated solely by the IP3 receptor, and Ca2+-sensitized IICR, but not CICR, generates Ca2+ waves and Ca2+ oscillations.

Original languageEnglish
Pages (from-to)251-255
Number of pages5
JournalScience
Volume257
Issue number5067
Publication statusPublished - 1992 Jul 10
Externally publishedYes

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Cite this

Miyazaki, S. I., Yuzaki, M., Nakada, K., Shirakawa, H., Nakanishi, S., Nakade, S., & Mikoshiba, K. (1992). Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs. Science, 257(5067), 251-255.