Blue-light irradiation up-regulates the ent-kaurene synthase gene and affects the avoidance response of protonemal growth in Physcomitrella patens

Sho Miyazaki, Hikaru Toyoshima, Masahiro Natsume, Masatoshi Nakajima, Hiroshi Kawaide

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Main conclusion: We report a novel physiological response to blue light in the moss Physcomitrella patens. Blue light regulates ent -kaurene biosynthesis and avoidance response to protonemal growth. Gibberellins (GAs) are a group of diterpene-type plant hormones biosynthesized from ent-kaurenoic acid via ent-kaurene. While the moss Physcomitrella patens has part of the GA biosynthetic pathway, from geranylgeranyl diphosphate to ent-kaurenoic acid, no GA is found in this species. Caulonemal differentiation in a P. patens mutant with a disrupted bifunctional ent-copalyl diphosphate synthase/ent-kaurene synthase (PpCPS/KS) gene is suppressed under red light, and is recovered by application of ent-kaurene and ent-kaurenoic acid. This indicates that derivatives of ent-kaurenoic acid, not GAs, might act as endogenous developmental regulators. Here, we found unique responses in the protonemal growth of P. patens under unilateral blue light, and these regulators were involved in the responses. When protonemata of the wild type were incubated under blue light, the chloronemal filaments grew in the opposite direction to the light source. Although this avoidance was not observed in the ent-kaurene deficient mutant, chloronemal growth toward a blue-light source in the mutant was suppressed by application of ent-kaurenoic acid, and the growth was rescued to that in the wild type. Expression analysis of the PpCPS/KS gene showed that the mRNA level under blue light was rapidly increased and was five times higher than under red light. These results suggest that regulators derived from ent-kaurenoic acid are strongly involved not only in the growth regulation of caulonemal differentiation under red light, but also in the light avoidance response of chloronemal growth under blue light. In particular, growth under blue light is regulated via the PpCPS/KS gene.

Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalPlanta
Volume240
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Bryopsida
Physcomitrella patens
kaurenoic acid
blue light
Up-Regulation
irradiation
Light
Growth
Kaurane Diterpenes
Genes
genes
red light
mutants
mosses and liverworts
Bryophyta
protonemata
ent-kaurene synthetase B
diterpenoids
gibberellins
plant hormones

Keywords

  • Blue-light signaling
  • Diterpene
  • ent-Kaurenoic acid
  • Gibberellin
  • Physcomitrella
  • Red-light signaling

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Medicine(all)

Cite this

Blue-light irradiation up-regulates the ent-kaurene synthase gene and affects the avoidance response of protonemal growth in Physcomitrella patens. / Miyazaki, Sho; Toyoshima, Hikaru; Natsume, Masahiro; Nakajima, Masatoshi; Kawaide, Hiroshi.

In: Planta, Vol. 240, No. 1, 01.01.2014, p. 117-124.

Research output: Contribution to journalArticle

Miyazaki, Sho ; Toyoshima, Hikaru ; Natsume, Masahiro ; Nakajima, Masatoshi ; Kawaide, Hiroshi. / Blue-light irradiation up-regulates the ent-kaurene synthase gene and affects the avoidance response of protonemal growth in Physcomitrella patens. In: Planta. 2014 ; Vol. 240, No. 1. pp. 117-124.
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