Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus

Masanori Matsuura, Mina Yamada, Yoko Saikawa, Kazuo Miyairi, Toshikatsu Okuno, Katsuhiro Konno, Jun'ichi Uenishi, Kimiko Hashimoto, Masaya Nakata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A toxic protein, called bolevenine, was isolated from the toxic mushroom Boletus venenatus based on its lethal effects on mice. On SDS-PAGE, in either the presence or absence of 2-mercaptoethanol, this protein showed a single band of ∼12 kDa. In contrast, based on gel filtration and MALDI-TOFMS, its relative molecular mass was estimated to be ∼30 kDa and ∼33 kDa, respectively, indicating that the protein consists of three identical subunits. This toxin exhibited its lethal activity following injection at 10 mg/kg into mice. The N-terminal amino acid sequence was determined up to 18, and found to be similar to the previously reported bolesatine, a toxic compound isolated from Boletus satanas.

Original languageEnglish
Pages (from-to)893-898
Number of pages6
JournalPhytochemistry
Volume68
Issue number6
DOIs
Publication statusPublished - 2007 Mar

Fingerprint

Boletus
Poisons
lethal genes
Proteins
proteins
Mercaptoethanol
Agaricales
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
mice
Molecular mass
toxic substances
mushrooms
Gel Chromatography
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
toxins
amino acid sequences
Gels
gels
molecular weight

Keywords

  • Boletaceae
  • Boletus venenatus
  • Bolevenine
  • Mushroom poisoning
  • Toxic protein
  • Trimeric protein

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery

Cite this

Matsuura, M., Yamada, M., Saikawa, Y., Miyairi, K., Okuno, T., Konno, K., ... Nakata, M. (2007). Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus. Phytochemistry, 68(6), 893-898. https://doi.org/10.1016/j.phytochem.2006.11.037

Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus. / Matsuura, Masanori; Yamada, Mina; Saikawa, Yoko; Miyairi, Kazuo; Okuno, Toshikatsu; Konno, Katsuhiro; Uenishi, Jun'ichi; Hashimoto, Kimiko; Nakata, Masaya.

In: Phytochemistry, Vol. 68, No. 6, 03.2007, p. 893-898.

Research output: Contribution to journalArticle

Matsuura, M, Yamada, M, Saikawa, Y, Miyairi, K, Okuno, T, Konno, K, Uenishi, J, Hashimoto, K & Nakata, M 2007, 'Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus', Phytochemistry, vol. 68, no. 6, pp. 893-898. https://doi.org/10.1016/j.phytochem.2006.11.037
Matsuura, Masanori ; Yamada, Mina ; Saikawa, Yoko ; Miyairi, Kazuo ; Okuno, Toshikatsu ; Konno, Katsuhiro ; Uenishi, Jun'ichi ; Hashimoto, Kimiko ; Nakata, Masaya. / Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus. In: Phytochemistry. 2007 ; Vol. 68, No. 6. pp. 893-898.
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