Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus

Masanori Matsuura, Mina Yamada, Yoko Saikawa, Kazuo Miyairi, Toshikatsu Okuno, Katsuhiro Konno, Jun'ichi Uenishi, Kimiko Hashimoto, Masaya Nakata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A toxic protein, called bolevenine, was isolated from the toxic mushroom Boletus venenatus based on its lethal effects on mice. On SDS-PAGE, in either the presence or absence of 2-mercaptoethanol, this protein showed a single band of ∼12 kDa. In contrast, based on gel filtration and MALDI-TOFMS, its relative molecular mass was estimated to be ∼30 kDa and ∼33 kDa, respectively, indicating that the protein consists of three identical subunits. This toxin exhibited its lethal activity following injection at 10 mg/kg into mice. The N-terminal amino acid sequence was determined up to 18, and found to be similar to the previously reported bolesatine, a toxic compound isolated from Boletus satanas.

Original languageEnglish
Pages (from-to)893-898
Number of pages6
JournalPhytochemistry
Volume68
Issue number6
DOIs
Publication statusPublished - 2007 Mar 1

Keywords

  • Boletaceae
  • Boletus venenatus
  • Bolevenine
  • Mushroom poisoning
  • Toxic protein
  • Trimeric protein

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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  • Cite this

    Matsuura, M., Yamada, M., Saikawa, Y., Miyairi, K., Okuno, T., Konno, K., Uenishi, J., Hashimoto, K., & Nakata, M. (2007). Bolevenine, a toxic protein from the Japanese toadstool Boletus venenatus. Phytochemistry, 68(6), 893-898. https://doi.org/10.1016/j.phytochem.2006.11.037