Bone Cell Biology Assessed by Microscopic Approach. Regulation of bone mineralization through the osteocyte lacuno-canalicular network

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Abstract

Osteocytes and their dendrites form a large network called the lacuno-canalicular system in mammalian bone. Osteocytes are believed to directly contribute to regulation of mineralization and demineralization in bone matrix, in addition to their indirect regulation of these processes through osteoblasts and osteoclasts. Not only can the location and shape of osteocyte lacunae and canaliculi be spatially visualized in isolated bone samples using synchrotron radiation technology, but differences in the degree of mineralization throughout the lacuno-canalicular system can be detected and quantified. Currently, comparable observation of the time course of these activities in vivo is technically challenging. This review provides an overview of non-dynamic quantitative analysis in the lacuno-canalicular system. Such analysis has the potential to become a methodological basis for investigating osteocyte-dependent direct regulation of mineralization in bone diseases.

Original languageEnglish
Pages (from-to)1461-1466
Number of pages6
JournalClinical calcium
Volume25
Issue number10
Publication statusPublished - 2015 Oct 1

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Osteocytes
Physiologic Calcification
Cell Biology
Bone and Bones
Synchrotrons
Bone Matrix
Bone Diseases
Osteoclasts
Dendrites
Osteoblasts
Observation
Radiation
Technology

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Osteocytes and their dendrites form a large network called the lacuno-canalicular system in mammalian bone. Osteocytes are believed to directly contribute to regulation of mineralization and demineralization in bone matrix, in addition to their indirect regulation of these processes through osteoblasts and osteoclasts. Not only can the location and shape of osteocyte lacunae and canaliculi be spatially visualized in isolated bone samples using synchrotron radiation technology, but differences in the degree of mineralization throughout the lacuno-canalicular system can be detected and quantified. Currently, comparable observation of the time course of these activities in vivo is technically challenging. This review provides an overview of non-dynamic quantitative analysis in the lacuno-canalicular system. Such analysis has the potential to become a methodological basis for investigating osteocyte-dependent direct regulation of mineralization in bone diseases.",
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