Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets

Takayuki Mashimo, Yukio Sato, Daisuke Akita, Taku Toriumi, Shunsuke Namaki, Yumi Matsuzaki, Yoshiyuki Yonehara, Masaki Honda

Research output: Contribution to journalArticle

Abstract

Bone marrow-derived mesenchymal stem cells (BMMSCs) remain the most widely used source of osteogenic cells in bone tissue engineering research. A cell-based treatment for alveolar ridge augmentation has received attention as an alternative to bone grafting. In the present study, BMMSC transplantation into tooth extraction sockets of C57BL/6J mice was evaluated for alveolar ridge regeneration. The first right maxillary molars were extracted, and then BMMSCs (PDGFRα+ Sca-1+ CD45 TER119 cells) isolated from femoral and tibial bone marrow were immediately transplanted into the extraction sockets. A control group underwent the same procedure except for BMMSC transplantation. Bone formation in the sockets was evaluated using micro-computed tomography and histological and immunohisto-chemical analyses. At 3 weeks, bone formation in the sockets was more advanced in the experimental group than in the control group. Histological analysis at 6 weeks after transplantation showed that the sockets in the experimental group also contained a greater quantity of bone marrow. Interestingly, socket bone mineral density was lower in the experimental group than in the control group at 6 weeks. These findings suggest that BMMSC transplantation accelerates bone healing and augments bone marrow formation in tooth extraction sockets.

Original languageEnglish
Pages (from-to)284-293
Number of pages10
JournalJournal of Oral Science
Volume61
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

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Tooth Socket
Tooth Extraction
Bone Regeneration
Mesenchymal Stromal Cells
Bone Marrow
Mesenchymal Stem Cell Transplantation
Osteogenesis
Control Groups
Alveolar Ridge Augmentation
Alveolar Process
Bone and Bones
Bone Transplantation
Tissue Engineering
Thigh
Inbred C57BL Mouse
Bone Density
Regeneration
Transplantation
Tomography

Keywords

  • Bone marrow
  • Bone regeneration
  • Dental socket
  • Mesenchymal stem cells
  • Tooth extraction

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets. / Mashimo, Takayuki; Sato, Yukio; Akita, Daisuke; Toriumi, Taku; Namaki, Shunsuke; Matsuzaki, Yumi; Yonehara, Yoshiyuki; Honda, Masaki.

In: Journal of Oral Science, Vol. 61, No. 2, 01.01.2019, p. 284-293.

Research output: Contribution to journalArticle

Mashimo, T, Sato, Y, Akita, D, Toriumi, T, Namaki, S, Matsuzaki, Y, Yonehara, Y & Honda, M 2019, 'Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets', Journal of Oral Science, vol. 61, no. 2, pp. 284-293. https://doi.org/10.2334/josnusd.18-0143
Mashimo, Takayuki ; Sato, Yukio ; Akita, Daisuke ; Toriumi, Taku ; Namaki, Shunsuke ; Matsuzaki, Yumi ; Yonehara, Yoshiyuki ; Honda, Masaki. / Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets. In: Journal of Oral Science. 2019 ; Vol. 61, No. 2. pp. 284-293.
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