Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets

Takayuki Mashimo, Yukio Sato, Daisuke Akita, Taku Toriumi, Shunsuke Namaki, Yumi Matsuzaki, Yoshiyuki Yonehara, Masaki Honda

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Abstract

Bone marrow-derived mesenchymal stem cells (BMMSCs) remain the most widely used source of osteogenic cells in bone tissue engineering research. A cell-based treatment for alveolar ridge augmentation has received attention as an alternative to bone grafting. In the present study, BMMSC transplantation into tooth extraction sockets of C57BL/6J mice was evaluated for alveolar ridge regeneration. The first right maxillary molars were extracted, and then BMMSCs (PDGFRα+ Sca-1+ CD45 TER119 cells) isolated from femoral and tibial bone marrow were immediately transplanted into the extraction sockets. A control group underwent the same procedure except for BMMSC transplantation. Bone formation in the sockets was evaluated using micro-computed tomography and histological and immunohisto-chemical analyses. At 3 weeks, bone formation in the sockets was more advanced in the experimental group than in the control group. Histological analysis at 6 weeks after transplantation showed that the sockets in the experimental group also contained a greater quantity of bone marrow. Interestingly, socket bone mineral density was lower in the experimental group than in the control group at 6 weeks. These findings suggest that BMMSC transplantation accelerates bone healing and augments bone marrow formation in tooth extraction sockets.

Original languageEnglish
Pages (from-to)284-293
Number of pages10
JournalJournal of Oral Science
Volume61
Issue number2
DOIs
Publication statusPublished - 2019 Jan 1

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Keywords

  • Bone marrow
  • Bone regeneration
  • Dental socket
  • Mesenchymal stem cells
  • Tooth extraction

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Mashimo, T., Sato, Y., Akita, D., Toriumi, T., Namaki, S., Matsuzaki, Y., Yonehara, Y., & Honda, M. (2019). Bone marrow-derived mesenchymal stem cells enhance bone marrow regeneration in dental extraction sockets. Journal of Oral Science, 61(2), 284-293. https://doi.org/10.2334/josnusd.18-0143