Bop encodes a muscle-restricted protein containing MYND and SET domains and is essential for cardiac differentiation and morphogenesis

Paul D. Gottlieb, Stephanie A. Pierce, Robert J. Sims, Hiroyuki Yamagishi, Elizabeth K. Weihe, June V. Harriss, Shanna D. Maika, William A. Kuziel, Heather L. King, Eric N. Olson, Osamu Nakagawa, Deepak Srivastava

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

Many transcription factors regulate specific temporal-spatial events during cardiac differentiation; however, the mechanisms that regulate such events are largely unknown. Using a modified subtractive hybridization method to identify specific genes that influence early cardiac development, we found that Bop is expressed specifically in cardiac and skeletal muscle precursors before differentiation of these lineages. Bop encodes a protein containing MYND and SET domains, which have been shown to regulate transcription by mediating distinct chromatin modifications. We show that m-Bop is a histone deacetylase-dependent transcriptional repressor. Targeted deletion of Bop in mice disrupted maturation of ventricular cardiomyocytes and interfered with formation of the right ventricle. Normal expression of Hand2, a transcription factor essential for right ventricular development, in cardiomyocyte precursors is dependent upon m-Bop. These results indicate that m-Bop is essential for cardiomyocyte differentiation and cardiac morphogenesis.

Original languageEnglish
Pages (from-to)25-32
Number of pages8
JournalNature Genetics
Volume31
Issue number1
DOIs
Publication statusPublished - 2002 May
Externally publishedYes

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Muscle Proteins
Morphogenesis
Cardiac Myocytes
Transcription Factors
Histone Deacetylases
Chromatin
Heart Ventricles
Myocardium
Skeletal Muscle
Genes
Proteins

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Bop encodes a muscle-restricted protein containing MYND and SET domains and is essential for cardiac differentiation and morphogenesis. / Gottlieb, Paul D.; Pierce, Stephanie A.; Sims, Robert J.; Yamagishi, Hiroyuki; Weihe, Elizabeth K.; Harriss, June V.; Maika, Shanna D.; Kuziel, William A.; King, Heather L.; Olson, Eric N.; Nakagawa, Osamu; Srivastava, Deepak.

In: Nature Genetics, Vol. 31, No. 1, 05.2002, p. 25-32.

Research output: Contribution to journalArticle

Gottlieb, PD, Pierce, SA, Sims, RJ, Yamagishi, H, Weihe, EK, Harriss, JV, Maika, SD, Kuziel, WA, King, HL, Olson, EN, Nakagawa, O & Srivastava, D 2002, 'Bop encodes a muscle-restricted protein containing MYND and SET domains and is essential for cardiac differentiation and morphogenesis', Nature Genetics, vol. 31, no. 1, pp. 25-32. https://doi.org/10.1038/ng866
Gottlieb, Paul D. ; Pierce, Stephanie A. ; Sims, Robert J. ; Yamagishi, Hiroyuki ; Weihe, Elizabeth K. ; Harriss, June V. ; Maika, Shanna D. ; Kuziel, William A. ; King, Heather L. ; Olson, Eric N. ; Nakagawa, Osamu ; Srivastava, Deepak. / Bop encodes a muscle-restricted protein containing MYND and SET domains and is essential for cardiac differentiation and morphogenesis. In: Nature Genetics. 2002 ; Vol. 31, No. 1. pp. 25-32.
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