Both rejection and tolerance of allografts can occur in the absence of secondary lymphoid tissues

Cavit D. Kant, Yoshinobu Akiyama, Katsunori Tanaka, Susan Shea, Yohei Yamada, Sarah E. Connolly, Jose Marino, Georges Tocco, Gilles Benichou

Research output: Contribution to journalArticle

Abstract

In this study, we showed that aly/aly mice, which are devoid of lymph nodes and Peyer's patches, acutely rejected fully allogeneic skin and heart grafts. They mounted potent inflammatory direct alloresponses but failed to develop indirect alloreactivity after transplantation. Remarkably, skin allografts also were rejected acutely by splenectomized aly/aly (aly/aly-spl-) mice devoid of all secondary lymphoid organs. In these recipients, the rejection was mediated by alloreactive CD8+ T cells presumably primed in the bone marrow. In contrast, cardiac transplants were not rejected by aly/aly-spl- mice. Actually, aly/aly-spl- mice that spontaneously accepted a heart allotransplant and displayed donor-specific tolerance also accepted skin grafts from the same, but not a third-party, donor via a mechanism involving CD4+ regulatory T cells producing IL-10 cytokine. Therefore, direct priming of alloreactive T cells, as well as rejection and regulatory tolerance of allogeneic transplants, can occur in recipient mice lacking secondary lymphoid organs.

Original languageEnglish
Pages (from-to)1364-1371
Number of pages8
JournalJournal of Immunology
Volume194
Issue number3
DOIs
Publication statusPublished - 2015 Feb 1
Externally publishedYes

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Transplantation Tolerance
Lymphoid Tissue
Transplants
Skin
T-Lymphocytes
Peyer's Patches
Regulatory T-Lymphocytes
Interleukin-10
Allografts
Transplantation
Lymph Nodes
Bone Marrow
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Kant, C. D., Akiyama, Y., Tanaka, K., Shea, S., Yamada, Y., Connolly, S. E., ... Benichou, G. (2015). Both rejection and tolerance of allografts can occur in the absence of secondary lymphoid tissues. Journal of Immunology, 194(3), 1364-1371. https://doi.org/10.4049/jimmunol.1401157

Both rejection and tolerance of allografts can occur in the absence of secondary lymphoid tissues. / Kant, Cavit D.; Akiyama, Yoshinobu; Tanaka, Katsunori; Shea, Susan; Yamada, Yohei; Connolly, Sarah E.; Marino, Jose; Tocco, Georges; Benichou, Gilles.

In: Journal of Immunology, Vol. 194, No. 3, 01.02.2015, p. 1364-1371.

Research output: Contribution to journalArticle

Kant, CD, Akiyama, Y, Tanaka, K, Shea, S, Yamada, Y, Connolly, SE, Marino, J, Tocco, G & Benichou, G 2015, 'Both rejection and tolerance of allografts can occur in the absence of secondary lymphoid tissues', Journal of Immunology, vol. 194, no. 3, pp. 1364-1371. https://doi.org/10.4049/jimmunol.1401157
Kant, Cavit D. ; Akiyama, Yoshinobu ; Tanaka, Katsunori ; Shea, Susan ; Yamada, Yohei ; Connolly, Sarah E. ; Marino, Jose ; Tocco, Georges ; Benichou, Gilles. / Both rejection and tolerance of allografts can occur in the absence of secondary lymphoid tissues. In: Journal of Immunology. 2015 ; Vol. 194, No. 3. pp. 1364-1371.
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