Bottom-up genome assembly using the Bacillus subtilis genome vector

Mitsuhiro Itaya, Kyoko Fujita, Azusa Kuroki, Kenji Tsuge

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

Original languageEnglish
Pages (from-to)41-43
Number of pages3
JournalNature Methods
Volume5
Issue number1
DOIs
Publication statusPublished - 2008 Jan

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Bacilli
Bacillus subtilis
Genes
Genome
Synthetic Biology
Mitochondria
Systems Biology
Biological Science Disciplines
Chloroplasts
DNA
Research

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Bottom-up genome assembly using the Bacillus subtilis genome vector. / Itaya, Mitsuhiro; Fujita, Kyoko; Kuroki, Azusa; Tsuge, Kenji.

In: Nature Methods, Vol. 5, No. 1, 01.2008, p. 41-43.

Research output: Contribution to journalArticle

Itaya, M, Fujita, K, Kuroki, A & Tsuge, K 2008, 'Bottom-up genome assembly using the Bacillus subtilis genome vector', Nature Methods, vol. 5, no. 1, pp. 41-43. https://doi.org/10.1038/nmeth1143
Itaya, Mitsuhiro ; Fujita, Kyoko ; Kuroki, Azusa ; Tsuge, Kenji. / Bottom-up genome assembly using the Bacillus subtilis genome vector. In: Nature Methods. 2008 ; Vol. 5, No. 1. pp. 41-43.
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