Brachyury-downstream gene sets in a chordate, Ciona intestinalis

Integrating notochord specification, morphogenesis and chordate evolution

Kohji Hotta, Hiroki Takahashi, Nori Satoh, Takashi Gojobori

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

In vertebrates, Brachyury, a T-box transcription factor gene, seems to have a dual role in the differentiation of axial midline mesoderm cells into notochord and gastrulation cell movements regulated by non-canonical Wnt/planar cell polarity (Wnt/PCP) signaling. To understand the function of Brachyury-downstream genes in chordate embryos, from a series of our survey on differential expression, including subtractive hybridization, dot-blot assays, EST sequences and the expression patterns in whole-mount in situ hybridization at embryonic stages, we developed a knowledge database called "CINOBI: Ciona Notochord and Brachyury-downstream gene Index" to create comprehensive catalogues of Brachyury-downstream gene sets in Ciona intestinalis. Combining genome and large-scale cDNA data, we were able to characterize 450 non-redundant Brachyury-downstream genes: Twenty-four genes were newly annotated as notochord-expressed genes. Several genes are components of signaling pathways such as Wnt/PCP, NfκB and TGF-β signaling. We propose that Brachyury is linked to these pathways regulating the expression of each component, and such a regulatory mechanism might be conserved among chordates.

Original languageEnglish
Pages (from-to)37-51
Number of pages15
JournalEvolution and Development
Volume10
Issue number1
DOIs
Publication statusPublished - 2008 Jan

Fingerprint

Ciona intestinalis
Chordata
Notochord
morphogenesis
Morphogenesis
gene
Genes
genes
Cell Polarity
Gene Components
Gastrulation
Expressed Sequence Tags
Mesoderm
suppression subtractive hybridization
Brachyury protein
chordate
Cell Movement
In Situ Hybridization
Vertebrates
cell movement

ASJC Scopus subject areas

  • Genetics
  • Embryology
  • Developmental Biology
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Genetics(clinical)
  • Agricultural and Biological Sciences(all)

Cite this

Brachyury-downstream gene sets in a chordate, Ciona intestinalis : Integrating notochord specification, morphogenesis and chordate evolution. / Hotta, Kohji; Takahashi, Hiroki; Satoh, Nori; Gojobori, Takashi.

In: Evolution and Development, Vol. 10, No. 1, 01.2008, p. 37-51.

Research output: Contribution to journalArticle

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