TY - JOUR
T1 - Brain natriuretic peptide is a novel cardiac hormone
AU - Saito, Yoshihiko
AU - Nakao, Kazuwa
AU - Itoh, Hiroshi
AU - Yamada, Takayuki
AU - Mukoyama, Masashi
AU - Arai, Hiroshi
AU - Hosoda, Kiminori
AU - Shirakami, Gotaro
AU - Suga, Shin ichi
AU - Minamino, Naoto
AU - Kangawa, Kenji
AU - Matsuo, Hisayuki
AU - Imura, Hiroo
PY - 1989/1/31
Y1 - 1989/1/31
N2 - Using a radioimmunoassay for brain natriuretic peptide (BNP), we have measured levels of BNP-like immunoreactivity (-LI) in extract of the porcine heart, in perfusate from the isolated porcine heart and in porcine plasma. BNP-LI was detected in the extract of the atrium, though no detectable amount of BNP-LI (more than 1 ng/g) was present in the ventricle. The BNP-LI level in the porcine atrium was 148.7 ± 23.3 ng/g. BNP-LI was also detected in the perfusate from the heart. Basal secretory rate of BNP was 3.18 ± 0.76 ng/min. Moreover, BNP-LI was detected in porcine plasma at the concentration of 4.2 ± 1.3 pg/ml. Gel filtration studies showed that BNP is present in the atrium as a large molecule and is secreted into the circulation as a small molecule. The percentage of BNP-LI to atrial natriuretic peptide (ANP)-LI was almost the same among the extract, the perfusate and the plasma (2-3 percent). These results indicate that BNP is synthesized in and is secreted into the circulation from the heart in a similar fashion as ANP.
AB - Using a radioimmunoassay for brain natriuretic peptide (BNP), we have measured levels of BNP-like immunoreactivity (-LI) in extract of the porcine heart, in perfusate from the isolated porcine heart and in porcine plasma. BNP-LI was detected in the extract of the atrium, though no detectable amount of BNP-LI (more than 1 ng/g) was present in the ventricle. The BNP-LI level in the porcine atrium was 148.7 ± 23.3 ng/g. BNP-LI was also detected in the perfusate from the heart. Basal secretory rate of BNP was 3.18 ± 0.76 ng/min. Moreover, BNP-LI was detected in porcine plasma at the concentration of 4.2 ± 1.3 pg/ml. Gel filtration studies showed that BNP is present in the atrium as a large molecule and is secreted into the circulation as a small molecule. The percentage of BNP-LI to atrial natriuretic peptide (ANP)-LI was almost the same among the extract, the perfusate and the plasma (2-3 percent). These results indicate that BNP is synthesized in and is secreted into the circulation from the heart in a similar fashion as ANP.
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U2 - 10.1016/S0006-291X(89)80056-7
DO - 10.1016/S0006-291X(89)80056-7
M3 - Article
C2 - 2521788
AN - SCOPUS:0024581941
SN - 0006-291X
VL - 158
SP - 360
EP - 368
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -