Brain natriuretic peptide is a novel cardiac hormone

Yoshihiko Saito, Kazuwa Nakao, Hiroshi Itoh, Takayuki Yamada, Masashi Mukoyama, Hiroshi Arai, Kiminori Hosoda, Gotaro Shirakami, Shin ichi Suga, Naoto Minamino, Kenji Kangawa, Hisayuki Matsuo, Hiroo Imura

Research output: Contribution to journalArticle

193 Citations (Scopus)

Abstract

Using a radioimmunoassay for brain natriuretic peptide (BNP), we have measured levels of BNP-like immunoreactivity (-LI) in extract of the porcine heart, in perfusate from the isolated porcine heart and in porcine plasma. BNP-LI was detected in the extract of the atrium, though no detectable amount of BNP-LI (more than 1 ng/g) was present in the ventricle. The BNP-LI level in the porcine atrium was 148.7 ± 23.3 ng/g. BNP-LI was also detected in the perfusate from the heart. Basal secretory rate of BNP was 3.18 ± 0.76 ng/min. Moreover, BNP-LI was detected in porcine plasma at the concentration of 4.2 ± 1.3 pg/ml. Gel filtration studies showed that BNP is present in the atrium as a large molecule and is secreted into the circulation as a small molecule. The percentage of BNP-LI to atrial natriuretic peptide (ANP)-LI was almost the same among the extract, the perfusate and the plasma (2-3 percent). These results indicate that BNP is synthesized in and is secreted into the circulation from the heart in a similar fashion as ANP.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume158
Issue number2
DOIs
Publication statusPublished - 1989 Jan 31
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Saito, Y., Nakao, K., Itoh, H., Yamada, T., Mukoyama, M., Arai, H., Hosoda, K., Shirakami, G., Suga, S. I., Minamino, N., Kangawa, K., Matsuo, H., & Imura, H. (1989). Brain natriuretic peptide is a novel cardiac hormone. Biochemical and Biophysical Research Communications, 158(2), 360-368. https://doi.org/10.1016/S0006-291X(89)80056-7