Brain nitrite production during global ischemia and reperfusion: An in vivo microdialysis study

Mamoru Shibata, Nobuo Araki, Junichi Hamada, Takahiro Sasaki, Kunio Shimazu, Yasuo Fukuuchi

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Nitric oxide (NO) is considered to be associated with the pathogenesis of cerebral ischemic injury. In the present study, NO production was continuously monitored employing in vivo microdialysis. A microdialysis probe was inserted into the striatum. Levels of the major NO metabolite, NO2- in the dialysate were determined using the Griess reaction. Rats were subjected to global cerebral ischemia produced by occlusion of both common carotid arteries together with induced hypotension. Cerebral ischemia induced a decrease in NO production, which was interrupted by a transient increase in NO synthesis. This increment was abolished in the presence of a NO synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), suggesting that NO synthase activity is transiently activated during ischemia. Following reperfusion, NO synthesis was enhanced. To our knowledge, this is the first report concerning the continuous temporal profile of NO production during global cerebral ischemia and reperfusion.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalBrain Research
Volume734
Issue number1-2
DOIs
Publication statusPublished - 1996 Sep 23

Fingerprint

Microdialysis
Nitrites
Reperfusion
Nitric Oxide
Ischemia
Brain
Brain Ischemia
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Controlled Hypotension
Common Carotid Artery
Dialysis Solutions
Wounds and Injuries

Keywords

  • Cerebral ischemia
  • Griess reaction
  • Microdialysis, in vivo
  • Nitric oxide
  • Nitrite
  • Striatum

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brain nitrite production during global ischemia and reperfusion : An in vivo microdialysis study. / Shibata, Mamoru; Araki, Nobuo; Hamada, Junichi; Sasaki, Takahiro; Shimazu, Kunio; Fukuuchi, Yasuo.

In: Brain Research, Vol. 734, No. 1-2, 23.09.1996, p. 86-90.

Research output: Contribution to journalArticle

Shibata, Mamoru ; Araki, Nobuo ; Hamada, Junichi ; Sasaki, Takahiro ; Shimazu, Kunio ; Fukuuchi, Yasuo. / Brain nitrite production during global ischemia and reperfusion : An in vivo microdialysis study. In: Brain Research. 1996 ; Vol. 734, No. 1-2. pp. 86-90.
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