Brain transcriptome analysis links deficiencies of stress-responsive proteins to the pathomechanism of Kii ALS/PDC

Satoru Morimoto, Mitsuru Ishikawa, Hirotaka Watanabe, Miho Isoda, Masaki Takao, Shiho Nakamura, Fumiko Ozawa, Yoshifumi Hirokawa, Shigeki Kuzuhara, Hideyuki Okano, Yasumasa Kokubo

Research output: Contribution to journalArticle

Abstract

Amyotrophic lateral sclerosis and Parkinsonism-dementia complex (ALS/PDC) is a unique endemic neurodegenerative disease, with high-incidence foci in Kii Peninsula, Japan. To gather new insights into the pathological mechanisms underlying Kii ALS/PDC, we performed transcriptome analyses of patient brains. We prepared frozen brains from three individuals without neurodegenerative diseases, three patients with Alzheimer’s disease, and 21 patients with Kii ALS/PDC, and then acquired microarray data from cerebral gray and white matter tissues. Microarray results revealed that expression levels of genes associated with heat shock proteins, DNA binding/damage, and senescence were significantly altered in patients with ALS/PDC compared with healthy individuals. The RNA expression pattern observed for ALS-type brains was similar to that of PDC-type brains. Additionally, pathway and network analyses indicated that the molecular mechanism underlying ALS/PDC may be associated with oxidative phosphorylation of mitochondria, ribosomes, and the synaptic vesicle cycle; in particular, upstream regulators of these mechanisms may be found in synapses and during synaptic trafficking. Furthermore, phenotypic differences between ALS-type and PDC-type were observed, based on HLA haplotypes. In conclusion, determining the relationship between stress-responsive proteins, synaptic dysfunction, and the pathogenesis of ALS/PDC in the Kii peninsula may provide new understanding of this mysterious disease.

Original languageEnglish
Article number423
JournalAntioxidants
Volume9
Issue number5
DOIs
Publication statusPublished - 2020 May

Keywords

  • Amyotrophic lateral sclerosis (ALS)
  • HLA typing
  • Heat shock proteins
  • Kii peninsula
  • Mitochondria
  • Parkinsonism-dementia complex (PDC)
  • Stress-responsive proteins
  • Synaptic trafficking
  • Transcriptome analysis

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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    Morimoto, S., Ishikawa, M., Watanabe, H., Isoda, M., Takao, M., Nakamura, S., Ozawa, F., Hirokawa, Y., Kuzuhara, S., Okano, H., & Kokubo, Y. (2020). Brain transcriptome analysis links deficiencies of stress-responsive proteins to the pathomechanism of Kii ALS/PDC. Antioxidants, 9(5), [423]. https://doi.org/10.3390/antiox9050423