Broad distribution of the JC virus receptor contrasts with a marked cellular restriction of virus replication

Satoko Suzuki, Hirofumi Sawa, Rika Komagome, Yasuko Orba, Misato Yamada, Yuki Okada, Yusuke Ishida, Hiroshi Nishihara, Shinya Tanaka, Kazuo Nagashima

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

To investigate the early events of JC virus (JCV) infection, including attachment, penetration, transport to the nuclei, and replication of the virus, we analyzed the susceptibility of 15 different cell lines to infection using a semiquantitative polymerase chain reaction (PCR) assay, in situ hybridization, laser scanning confocal microscopy, and a viral replication assay. The cell lines examined were human permissive and nonpermissive cells as well as cells of monkey and mouse origin. JCV entry into the nuclei of the all cell lines was observed within 10 min after inoculation, demonstrating that the virus receptor is widely distributed among mammalian cells. Inhibition of viral entry by an anti-JCV VP1 antibody and sialidase treatment to remove sialic acid residues, which are considered a candidate for the JCV receptor, suggested that VP1 may interact with the cellular surface sialic acid. In addition, chlorpromazine, a clathrin-dependent pathway inhibitor, significantly suppressed entry of JCV into nuclei. In spite of the broad spectrum of cells susceptible to JCV entry, replication of the virus occurred exclusively in human neuroblastoma cell lines. These results suggest that whereas JCV can enter a wide variety of cell types and localize to the nuclei, cell-specific intranuclear mechanisms are required for virus replication.

Original languageEnglish
Pages (from-to)100-112
Number of pages13
JournalVirology
Volume286
Issue number1
DOIs
Publication statusPublished - 2001 Jul 20
Externally publishedYes

Fingerprint

JC Virus
Virus Receptors
Virus Replication
Cell Line
Virus Internalization
N-Acetylneuraminic Acid
Clathrin
Chlorpromazine
Neuraminidase
Virus Diseases
Cell Nucleus
Neuroblastoma
Confocal Microscopy
Haplorhini
In Situ Hybridization
Viruses
Polymerase Chain Reaction
Antibodies
Infection

Keywords

  • JC virus
  • Semiquantitative PCR
  • Viral entry
  • Virus-like particle

ASJC Scopus subject areas

  • Virology

Cite this

Suzuki, S., Sawa, H., Komagome, R., Orba, Y., Yamada, M., Okada, Y., ... Nagashima, K. (2001). Broad distribution of the JC virus receptor contrasts with a marked cellular restriction of virus replication. Virology, 286(1), 100-112. https://doi.org/10.1006/viro.2001.0972

Broad distribution of the JC virus receptor contrasts with a marked cellular restriction of virus replication. / Suzuki, Satoko; Sawa, Hirofumi; Komagome, Rika; Orba, Yasuko; Yamada, Misato; Okada, Yuki; Ishida, Yusuke; Nishihara, Hiroshi; Tanaka, Shinya; Nagashima, Kazuo.

In: Virology, Vol. 286, No. 1, 20.07.2001, p. 100-112.

Research output: Contribution to journalArticle

Suzuki, S, Sawa, H, Komagome, R, Orba, Y, Yamada, M, Okada, Y, Ishida, Y, Nishihara, H, Tanaka, S & Nagashima, K 2001, 'Broad distribution of the JC virus receptor contrasts with a marked cellular restriction of virus replication', Virology, vol. 286, no. 1, pp. 100-112. https://doi.org/10.1006/viro.2001.0972
Suzuki, Satoko ; Sawa, Hirofumi ; Komagome, Rika ; Orba, Yasuko ; Yamada, Misato ; Okada, Yuki ; Ishida, Yusuke ; Nishihara, Hiroshi ; Tanaka, Shinya ; Nagashima, Kazuo. / Broad distribution of the JC virus receptor contrasts with a marked cellular restriction of virus replication. In: Virology. 2001 ; Vol. 286, No. 1. pp. 100-112.
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