Bronchiolar epithelial catalase is diminished in smokers with mild COPD

Tomoko Betsuyaku, Satoshi Fuke, Takashi Inomata, Kichizo Kaga, Toshiaki Morikawa, Nao Odajima, Tracy Adair-Kirk, Masaharu Nishimura

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study aimed to investigate bronchiolar catalase expression and its relationship with smoking and/or chronic obstructive pulmonary disease (COPD) in humans and to determine the dynamic change of bronchiolar catalase expression in response to cigarette smoke in mice. Lung tissue was obtained from 36 subjects undergoing surgery for peripheral tumours, consisting of lifelong nonsmokers and smokers with or without COPD. Male C57BL/6 mice were subjected to cigarette smoke exposure for up to 3 months followed by a 28-day cessation period. We quantified bronchiolar catalase mRNA using laser capture microdissection and quantitative reverse transcription-polymerase chain reaction. C22 club cells (Clara cells) in culture were exposed to cigarette smoke extract and monitored for viability when catalase expression was decreased by siRNA. Catalase was decreased at mRNA and protein levels in bronchiolar epithelium in smokers with COPD. In mice, bronchiolar catalase is temporarily upregulated at 1 day after cigarette smoke exposure but is downregulated by repeated cigarette smoke exposure, and is not restored long after withdrawal once emphysema is developed. Decreasing catalase expression in C22 cells resulted in greater cigarette smoke extract-induced cell death. Bronchiolar catalase reduction is associated with COPD. Regulation of catalase depends on the duration of cigarette smoke exposure, and plays a critical role for protection against cigarette smoke-induced cell damage.

Original languageEnglish
Pages (from-to)42-53
Number of pages12
JournalEuropean Respiratory Journal
Volume42
Issue number1
DOIs
Publication statusPublished - 2013 Jul 1

Fingerprint

Catalase
Chronic Obstructive Pulmonary Disease
Smoke
Tobacco Products
Laser Capture Microdissection
Messenger RNA
Emphysema
Inbred C57BL Mouse
Small Interfering RNA
Reverse Transcription
Cell Death
Down-Regulation
Epithelium
Cell Culture Techniques
Smoking
Polymerase Chain Reaction
Lung

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Betsuyaku, T., Fuke, S., Inomata, T., Kaga, K., Morikawa, T., Odajima, N., ... Nishimura, M. (2013). Bronchiolar epithelial catalase is diminished in smokers with mild COPD. European Respiratory Journal, 42(1), 42-53. https://doi.org/10.1183/09031936.00058912

Bronchiolar epithelial catalase is diminished in smokers with mild COPD. / Betsuyaku, Tomoko; Fuke, Satoshi; Inomata, Takashi; Kaga, Kichizo; Morikawa, Toshiaki; Odajima, Nao; Adair-Kirk, Tracy; Nishimura, Masaharu.

In: European Respiratory Journal, Vol. 42, No. 1, 01.07.2013, p. 42-53.

Research output: Contribution to journalArticle

Betsuyaku, T, Fuke, S, Inomata, T, Kaga, K, Morikawa, T, Odajima, N, Adair-Kirk, T & Nishimura, M 2013, 'Bronchiolar epithelial catalase is diminished in smokers with mild COPD', European Respiratory Journal, vol. 42, no. 1, pp. 42-53. https://doi.org/10.1183/09031936.00058912
Betsuyaku T, Fuke S, Inomata T, Kaga K, Morikawa T, Odajima N et al. Bronchiolar epithelial catalase is diminished in smokers with mild COPD. European Respiratory Journal. 2013 Jul 1;42(1):42-53. https://doi.org/10.1183/09031936.00058912
Betsuyaku, Tomoko ; Fuke, Satoshi ; Inomata, Takashi ; Kaga, Kichizo ; Morikawa, Toshiaki ; Odajima, Nao ; Adair-Kirk, Tracy ; Nishimura, Masaharu. / Bronchiolar epithelial catalase is diminished in smokers with mild COPD. In: European Respiratory Journal. 2013 ; Vol. 42, No. 1. pp. 42-53.
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