TY - GEN
T1 - Bubble-Assisted in-Situ Re-Formation of Artificial Bilayer
AU - Hashimoto, Izumi
AU - Sugiura, Hirotaka
AU - Osaki, Toshihisa
AU - Yamada, Tetsuya
AU - Mimura, Hisatoshi
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Publisher Copyright:
© 2020 IEEE.
PY - 2020/1
Y1 - 2020/1
N2 - This paper proposes a technique to reconstitute a lipid bilayer membrane by injecting an air bubble onto the raptured spot. Our group has been studying the artificial bilayer that can host membrane proteins and can be a fundamental to analyze their functions. The only drawback of the lipid bilayer formed at the interface of two water-in-oil droplets is its sustainability, namely, the bilayer often collapses and the droplets merges by the disturbance like self-exerted oscillation or sloshing. To overcome this issue, the newly proposed technique utilizes a bubble at approximately 4μL in volume. When the bubble is injected and retracted, the new artificial bilayer is reconstituted. The major advantage of this technique is the in-situ reconstitution of the bilayer during the electrical measurement of the membrane proteins. The proposed technique was successfully verified by confirming the emergence of nanopore membrane proteins.
AB - This paper proposes a technique to reconstitute a lipid bilayer membrane by injecting an air bubble onto the raptured spot. Our group has been studying the artificial bilayer that can host membrane proteins and can be a fundamental to analyze their functions. The only drawback of the lipid bilayer formed at the interface of two water-in-oil droplets is its sustainability, namely, the bilayer often collapses and the droplets merges by the disturbance like self-exerted oscillation or sloshing. To overcome this issue, the newly proposed technique utilizes a bubble at approximately 4μL in volume. When the bubble is injected and retracted, the new artificial bilayer is reconstituted. The major advantage of this technique is the in-situ reconstitution of the bilayer during the electrical measurement of the membrane proteins. The proposed technique was successfully verified by confirming the emergence of nanopore membrane proteins.
KW - Bubble
KW - Lipid bilayer
KW - Self-assembly
UR - http://www.scopus.com/inward/record.url?scp=85083264795&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85083264795&partnerID=8YFLogxK
U2 - 10.1109/MEMS46641.2020.9056202
DO - 10.1109/MEMS46641.2020.9056202
M3 - Conference contribution
AN - SCOPUS:85083264795
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 1022
EP - 1023
BT - 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
Y2 - 18 January 2020 through 22 January 2020
ER -