TY - GEN
T1 - Bubble-Assisted Re-Formation of Individual Lipid Bilayers in Arrayed Device
AU - Hashimoto, Izumi
AU - Osaki, Toshihisa
AU - Mimura, Hisatoshi
AU - Takamori, Sho
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Funding Information:
This work was partly supported by JST START Grant Number JPMJST1811, and by the Program for Building Regional Innovation Ecosystem of MEXT, Japan.
Publisher Copyright:
© 2023 IEEE.
PY - 2023
Y1 - 2023
N2 - This paper describes a method capable of individual and selective re-formation of ruptured BLMs (bilayer lipid membranes) by using air bubbles in arrayed devices. BLMs play an important role in membrane protein studies, biosensor applications, and drug screening. In such applications, data throughput is a key issue, but BLMs are easily ruptured by physical/electrical disturbance, which makes it difficult to operate a large BLM array. In this work, we propose a bubble-assisted BLM re-formation method: An aqueous phase is divided by a small air bubble covered with a lipid monolayer. After the bubble departed, the divided aqueous phases are brought into contact with the lipid monolayer, resulting in formation of BLM. This method enables selective BLM re-formation in a multichannel device. To verify individual and selective BLM re-formation using this method, we developed a 4ch bubble-assisted device and demonstrated repetitive BLM re-formation.
AB - This paper describes a method capable of individual and selective re-formation of ruptured BLMs (bilayer lipid membranes) by using air bubbles in arrayed devices. BLMs play an important role in membrane protein studies, biosensor applications, and drug screening. In such applications, data throughput is a key issue, but BLMs are easily ruptured by physical/electrical disturbance, which makes it difficult to operate a large BLM array. In this work, we propose a bubble-assisted BLM re-formation method: An aqueous phase is divided by a small air bubble covered with a lipid monolayer. After the bubble departed, the divided aqueous phases are brought into contact with the lipid monolayer, resulting in formation of BLM. This method enables selective BLM re-formation in a multichannel device. To verify individual and selective BLM re-formation using this method, we developed a 4ch bubble-assisted device and demonstrated repetitive BLM re-formation.
KW - Air bubble
KW - Bilayer lipid membrane
KW - Re-formation
UR - http://www.scopus.com/inward/record.url?scp=85149869314&partnerID=8YFLogxK
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U2 - 10.1109/MEMS49605.2023.10052315
DO - 10.1109/MEMS49605.2023.10052315
M3 - Conference contribution
AN - SCOPUS:85149869314
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 279
EP - 280
BT - 2023 IEEE 36th International Conference on Micro Electro Mechanical Systems, MEMS 2023
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 36th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2023
Y2 - 15 January 2023 through 19 January 2023
ER -