Abstract
Objective: To compare buoyant density and sedimentation kinetics of human immunodeficiency virus 1 (HIV-1) in two sperm-washing media, Percoll and Pureception. Design: Laboratory study. Setting: University hospital. Patient(s): None. Intervention(s): Buoyant density and sedimentation kinetics of HIV-1 particles (MOLT-4/LAI strain) were measured in Percoll and Pureception using isopyknic ultracentrifugation and continuous-density-gradient centrifugation. Main Outcome Measure(s): The HIV-1 particles were detected and semiquantified using a reverse transcription polymerase chain reaction (RT-PCR) for HIV-1 RNA. Result(s): Calculated buoyant density of HIV-1 was approximately 1.042 in both media in isopyknic centrifugation. However, most HIV-1 particles were found in fractions with specific gravity less than 1.04 in both media, even after 40 minutes of density-gradient centrifugation at 1,600 g. Small viral accumulations were observed at the bottom of the tube in Pureception density gradients. Conclusion(s): Although we found very high efficiency of HIV-1 removal using density-gradient centrifugation, a minute quantity of virus was found at the bottom of the gradient tube when Pureception was used as the medium.
Original language | English |
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Pages (from-to) | 1983-1987 |
Number of pages | 5 |
Journal | Fertility and Sterility |
Volume | 90 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2008 Nov |
Keywords
- HIV-1
- Percoll
- Pureception
- Sperm
- density-gradient centrifugation
- isopyknic centrifugation
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynaecology