c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model

Masahiro Shinoda, Motohide Shimazu, Satoshi Matsuda, Go Wakabayashi, Minoru Tanabe, Ken Hoshino, Shusaku Kamei, Shigeo Koyasu, Masaki Kitajima

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ischemia/reperfusion injuries are a major problem in liver resections and transplantations. Tumor necrosis factor-α has been widely investigated as a key mediator in the mechanism of ischemia/reperfusion injury. Upstream signal transduction mechanisms for tumor necrosis factor-α have not been well documented. Therefore, we assessed c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model, Male Wistar rats were subjected to 30 minutes of ischemia followed by reperfusion. Hepatic enzymes, histological examinations, microfluorographs, and tumor necrosis factor-α protein production (in the serum and liver tissue) were analyzed during the course of reperfusion. c-Jun N-terminal kinase activity was measured by a radioisotope kinase assay. Ischemia/reperfusion injuries were characterized by an elevation in hepatic enzyme, the histological degeneration of hepatocytes, and an increase in the number of nonviable cells. Moreover, increased endothelial-adherent leukocytes and tumor necrosis factor-α protein production were also observed. c-Jun N-terminal kinase activity at 60 minutes after reperfusion was 12.4 times higher than the pre-ischemia level. These results suggest that c-Jun N-terminal kinase may play some role in the mechanism of ischemia/reperfusion injuries.

Original languageEnglish
Pages (from-to)314-319
Number of pages6
JournalWound Repair and Regeneration
Volume10
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Warm Ischemia
JNK Mitogen-Activated Protein Kinases
Reperfusion Injury
Tumor Necrosis Factor-alpha
Liver
Reperfusion
Ischemia
Enzymes
Radioisotopes
Liver Transplantation
Wistar Rats
Hepatocytes
Signal Transduction
Proteins
Leukocytes
Phosphotransferases
Cell Count
Serum

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model. / Shinoda, Masahiro; Shimazu, Motohide; Matsuda, Satoshi; Wakabayashi, Go; Tanabe, Minoru; Hoshino, Ken; Kamei, Shusaku; Koyasu, Shigeo; Kitajima, Masaki.

In: Wound Repair and Regeneration, Vol. 10, No. 5, 2002, p. 314-319.

Research output: Contribution to journalArticle

Shinoda, M, Shimazu, M, Matsuda, S, Wakabayashi, G, Tanabe, M, Hoshino, K, Kamei, S, Koyasu, S & Kitajima, M 2002, 'c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model', Wound Repair and Regeneration, vol. 10, no. 5, pp. 314-319. https://doi.org/10.1046/j.1524-475X.2002.t01-1-10507.x
Shinoda, Masahiro ; Shimazu, Motohide ; Matsuda, Satoshi ; Wakabayashi, Go ; Tanabe, Minoru ; Hoshino, Ken ; Kamei, Shusaku ; Koyasu, Shigeo ; Kitajima, Masaki. / c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model. In: Wound Repair and Regeneration. 2002 ; Vol. 10, No. 5. pp. 314-319.
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