c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model

Masahiro Shinoda, Motohide Shimazu, Satoshi Matsuda, Go Wakabayashi, Minoru Tanabe, Ken Hoshino, Shusaku Kamei, Shigeo Koyasu, Masaki Kitajima

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9 Citations (Scopus)

Abstract

Ischemia/reperfusion injuries are a major problem in liver resections and transplantations. Tumor necrosis factor-α has been widely investigated as a key mediator in the mechanism of ischemia/reperfusion injury. Upstream signal transduction mechanisms for tumor necrosis factor-α have not been well documented. Therefore, we assessed c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model, Male Wistar rats were subjected to 30 minutes of ischemia followed by reperfusion. Hepatic enzymes, histological examinations, microfluorographs, and tumor necrosis factor-α protein production (in the serum and liver tissue) were analyzed during the course of reperfusion. c-Jun N-terminal kinase activity was measured by a radioisotope kinase assay. Ischemia/reperfusion injuries were characterized by an elevation in hepatic enzyme, the histological degeneration of hepatocytes, and an increase in the number of nonviable cells. Moreover, increased endothelial-adherent leukocytes and tumor necrosis factor-α protein production were also observed. c-Jun N-terminal kinase activity at 60 minutes after reperfusion was 12.4 times higher than the pre-ischemia level. These results suggest that c-Jun N-terminal kinase may play some role in the mechanism of ischemia/reperfusion injuries.

Original languageEnglish
Pages (from-to)314-319
Number of pages6
JournalWound Repair and Regeneration
Volume10
Issue number5
DOIs
Publication statusPublished - 2002 Nov 23

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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    Shinoda, M., Shimazu, M., Matsuda, S., Wakabayashi, G., Tanabe, M., Hoshino, K., Kamei, S., Koyasu, S., & Kitajima, M. (2002). c-Jun N-terminal kinase activation during warm hepatic ischemia/reperfusion injuries in a rat model. Wound Repair and Regeneration, 10(5), 314-319. https://doi.org/10.1046/j.1524-475X.2002.t01-1-10507.x