C-kit expression in spermatogonia damaged by doxorubicin exposure in mice

Reiko Tanigaki, Kou Sueoka, Hiroto Tajima, Akira Nakabayashi, Kenji Sato, Hironori Asada, Shingo Kato, Yoshihiko Hosoi, Yasunori Yoshimura

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Aim: The purpose of this study was to assess the relationship between chronically impaired spermatogenesis induced by exposing mice to doxorubicin (DXR) and expression of the infertility factor c-kit. Method: Eight-week-old male Institute for Cancer Research (ICR) mice were intraperitoneally treated with DXR (0.15 mg/kg, DXR group) or saline (0.15 mg/kg, control group) twice weekly for five weeks and were killed 14 weeks after initial exposure. The animals were sacrificed and bilateral testes were removed and weighed. The testes were stored for the mRNA assay and were fixed for immunohistochemistry. Some testicular samples were fixed in 10% formalin for histopathological examination. Results: Testicular weight (67.6 ± 9.7 mg, P < 0.05), sperm motility (18 ± 6.0%, P < 0.05) and the fertilization rate (2-to-16-cell embryos, 5%; P < 0.05) were significantly lower in the DXR group than in the control group. In the DXR group there was severe tissue damage from the spermatogonia onward, and the Sertoli cell ratio was lower in the DXR group than in the control group (38% vs. 9%, P < 0.05). In addition, there was a decrease in c-kit protein expression, and the amount of c-kit messenger ribonucleic acid (mRNA) expression according to a semiquantitative method was also decreased. Conclusion: Expression of c-kit in the mice with chronically impaired spermatogenesis induced by long-term, low-dose administration of DXR correlated with the decrease in the number of spermatogonia.

Original languageEnglish
Pages (from-to)692-700
Number of pages9
JournalJournal of Obstetrics and Gynaecology Research
Volume39
Issue number3
DOIs
Publication statusPublished - 2013 Mar

Fingerprint

Spermatogonia
Doxorubicin
Spermatogenesis
Control Groups
Testis
RNA
Proto-Oncogene Proteins c-kit
Sertoli Cells
Sperm Motility
Fertilization
Infertility
Formaldehyde
Embryonic Structures
Immunohistochemistry
Weights and Measures

Keywords

  • C-kit
  • Doxorubicin
  • Male infertility
  • Spermatogenesis
  • Spermatogonia

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

C-kit expression in spermatogonia damaged by doxorubicin exposure in mice. / Tanigaki, Reiko; Sueoka, Kou; Tajima, Hiroto; Nakabayashi, Akira; Sato, Kenji; Asada, Hironori; Kato, Shingo; Hosoi, Yoshihiko; Yoshimura, Yasunori.

In: Journal of Obstetrics and Gynaecology Research, Vol. 39, No. 3, 03.2013, p. 692-700.

Research output: Contribution to journalArticle

Tanigaki, R, Sueoka, K, Tajima, H, Nakabayashi, A, Sato, K, Asada, H, Kato, S, Hosoi, Y & Yoshimura, Y 2013, 'C-kit expression in spermatogonia damaged by doxorubicin exposure in mice', Journal of Obstetrics and Gynaecology Research, vol. 39, no. 3, pp. 692-700. https://doi.org/10.1111/j.1447-0756.2012.02006.x
Tanigaki, Reiko ; Sueoka, Kou ; Tajima, Hiroto ; Nakabayashi, Akira ; Sato, Kenji ; Asada, Hironori ; Kato, Shingo ; Hosoi, Yoshihiko ; Yoshimura, Yasunori. / C-kit expression in spermatogonia damaged by doxorubicin exposure in mice. In: Journal of Obstetrics and Gynaecology Research. 2013 ; Vol. 39, No. 3. pp. 692-700.
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AU - Tanigaki, Reiko

AU - Sueoka, Kou

AU - Tajima, Hiroto

AU - Nakabayashi, Akira

AU - Sato, Kenji

AU - Asada, Hironori

AU - Kato, Shingo

AU - Hosoi, Yoshihiko

AU - Yoshimura, Yasunori

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N2 - Aim: The purpose of this study was to assess the relationship between chronically impaired spermatogenesis induced by exposing mice to doxorubicin (DXR) and expression of the infertility factor c-kit. Method: Eight-week-old male Institute for Cancer Research (ICR) mice were intraperitoneally treated with DXR (0.15 mg/kg, DXR group) or saline (0.15 mg/kg, control group) twice weekly for five weeks and were killed 14 weeks after initial exposure. The animals were sacrificed and bilateral testes were removed and weighed. The testes were stored for the mRNA assay and were fixed for immunohistochemistry. Some testicular samples were fixed in 10% formalin for histopathological examination. Results: Testicular weight (67.6 ± 9.7 mg, P < 0.05), sperm motility (18 ± 6.0%, P < 0.05) and the fertilization rate (2-to-16-cell embryos, 5%; P < 0.05) were significantly lower in the DXR group than in the control group. In the DXR group there was severe tissue damage from the spermatogonia onward, and the Sertoli cell ratio was lower in the DXR group than in the control group (38% vs. 9%, P < 0.05). In addition, there was a decrease in c-kit protein expression, and the amount of c-kit messenger ribonucleic acid (mRNA) expression according to a semiquantitative method was also decreased. Conclusion: Expression of c-kit in the mice with chronically impaired spermatogenesis induced by long-term, low-dose administration of DXR correlated with the decrease in the number of spermatogonia.

AB - Aim: The purpose of this study was to assess the relationship between chronically impaired spermatogenesis induced by exposing mice to doxorubicin (DXR) and expression of the infertility factor c-kit. Method: Eight-week-old male Institute for Cancer Research (ICR) mice were intraperitoneally treated with DXR (0.15 mg/kg, DXR group) or saline (0.15 mg/kg, control group) twice weekly for five weeks and were killed 14 weeks after initial exposure. The animals were sacrificed and bilateral testes were removed and weighed. The testes were stored for the mRNA assay and were fixed for immunohistochemistry. Some testicular samples were fixed in 10% formalin for histopathological examination. Results: Testicular weight (67.6 ± 9.7 mg, P < 0.05), sperm motility (18 ± 6.0%, P < 0.05) and the fertilization rate (2-to-16-cell embryos, 5%; P < 0.05) were significantly lower in the DXR group than in the control group. In the DXR group there was severe tissue damage from the spermatogonia onward, and the Sertoli cell ratio was lower in the DXR group than in the control group (38% vs. 9%, P < 0.05). In addition, there was a decrease in c-kit protein expression, and the amount of c-kit messenger ribonucleic acid (mRNA) expression according to a semiquantitative method was also decreased. Conclusion: Expression of c-kit in the mice with chronically impaired spermatogenesis induced by long-term, low-dose administration of DXR correlated with the decrease in the number of spermatogonia.

KW - C-kit

KW - Doxorubicin

KW - Male infertility

KW - Spermatogenesis

KW - Spermatogonia

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